Topic
Collodion
About: Collodion is a research topic. Over the lifetime, 343 publications have been published within this topic receiving 12528 citations.
Papers published on a yearly basis
1 / 3
Papers
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Abstract: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed. Poly(A)+ and ribosomal RNAs transfer efficiently to nitrocellulose paper in high salt (3 M NaCl/0.3 M trisodium citrate) after denaturation with glyoxal and 50% (vol/vol) dimethyl sulfoxide. RNA also binds to nitrocellulose after treatment with methylmercuric hydroxide. The method is sensitive: about 50 pg of specific mRNA per band is readily detectable after hybridization with high specific activity probes (10(8) cpm/microgram). The RNA is stably bound to the nitrocellulose paper by this procedure, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity. The use of nitrocellulose paper for the analysis of RNA by blot hybridization has several advantages over the use of activated paper (diazobenzyloxymethyl-paper). The method is simple, inexpensive, reproducible, and sensitive. In addition, denaturation of DNA with glyoxal and dimethyl sulfoxide promotes transfer and retention of small DNAs (100 nucleotides and larger) to nitrocellulose paper. A related method is also described for dotting RNA and DNA directly onto nitrocellulose paper treated with a high concentration of salt; under these conditions denatured DNA of less than 200 nucleotides is retained and hybridizes efficiently.
8,618 citations
TL;DR: A staining technique, using Ponceau S in very mild conditions, by which proteins can be visualized on nitrocellulose replicas without being permanently fixed to the membrane itself, thus allowing subsequent procedures such as immunoblotting or preparative elution of the proteins to be performed.
413 citations
TL;DR: A very sensitive method for the detection of antigen-antibody complexes on nitrocellulose paper immunoblots is described and the improved of this method is the result of the use of the detergent Tween 20 in blocking the nonspecific binding of the antibodies to the nitrocells.
335 citations
TL;DR: In this article, a series of monodisperse polystyrene latexes has been prepared by carefully controlled emulsion polymerizations. The particle diameters of three of these latexes were determined by electron microscopy.
Abstract: A series of monodisperse polystyrene latexes has been prepared by carefully controlled emulsion polymerizations. The particle diameters of three of these latexes were determined by electron microscopy. The latex particles were dispersed on collodion membranes supported by copper grids. The microscopes had previously been calibrated with collodion replicas of a 30 000 line/inch diffraction grating. In general, the reproducibility of measurements from many photographic exposures was good; however, a few exposures yielded particle diameters considerably higher than the averages.An investigation of this technique indicated that: (1) polystyrene latex particle diameters (collodion membranes—copper grid supports) increased considerably on electron irradiation, (2) collodion diffraction grating replicas (copper grid supports) shrank slightly on electron irradiation, and (3) the magnification of the electron microscope varied slightly from exposure to exposure. It was found that variations of particle diameter re...
179 citations
161 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 6 |
| 2024 | 6 |
| 2023 | 14 |
| 2022 | 18 |
| 2021 | 3 |
| 2020 | 4 |