TL;DR: When surveying the karyotype diversity of European loaches of the genus Cobitis to identify species involved in hybrid polyploid complexes, an extensive polymorphism in number and location of NORs was discovered in C. vardarensis, suggesting that ribosomal sites are associated with GC-rich DNA.
Abstract: When surveying the karyotype diversity of European loaches of the genus Cobitis to identify species involved in hybrid polyploid complexes, an extensive polymorphism in number and location of NORs was discovered in C. vardarensis using Ag-staining, C-banding, CMA3-fluorescence and fluorescence in situ hybridization (FISH). This species had 2n = 50, the karyotype contained 13 pairs of metacentric, 10 pairs of submetacentric and two pairs of subtelocentric chromosomes. The NOR-bearing chromosomes included one medium-sized metacentric pair with a large CMA3-positive heterochromatic pericentromeric block, one small metacentric as well as one large submetacentric pairs. Ribosomal sites were always located in telomeres of these chromosomes. Each of the pair of NOR-bearing chromosomes occurred in three variants - (1) presence and/or (2) absence of NORs on both homologues and (3) heterozygous combination where only one of the homologues bears NORs. Altogether, 10 different NOR cytotypes from 27 theoretically possible ones were discovered among 20 indviduals examined. The number of NORs ranged from two to five per specimen. The results regarding the number and locations of NORs as revealed by banding techniques were confirmed using FISH with rDNA probe. NOR sites were of CMA3-positive, suggesting that ribosomal sites are associated with GC-rich DNA. Very similar structural polymorphism with multiple NORs is expressed in the Danubian loach C. elongatoides indicating a close relationship between both species.
TL;DR: Assessment of the abundance, food and microhabitat use of Proterorhinus marmoratus and Cobitis vardarensis during both day and night in two small temperate streams of the Black Sea region revealed significantly higher relative densities at night than during the day.
Abstract: We examined the abundance, food and microhabitat use of Proterorhinus marmoratus (Gobiidae) and Cobitis vardarensis (Cobitidae) during both day and night in two small temperate streams of the Black Sea region (North-West Turkey) between May and June 2005. Drift sampling catch per unit effort revealed significantly higher relative densities at night than during the day. Of the 110 fish captured during the seven sampling periods, 77 (88%) were C. vardarensis with the Býcký Stream having the most fish specimens both in frequency of occurrence and relative density. The main environmental variables that characterized the overall microhabitat use were velocity, light, depth, and proportions of cobbles. The fullness frequency of the gut contents was at their peak between 22:00 and 04:00 a.m and the most active feeding time was usually after midnight. Surface insects composed mainly of chirinomid larvae were the dominant food item for both species in the streams during the sampling period. Further studies are needed at multiple spatial scales to assess how habitat use and feeding habits varies with fluctuating population densities.
TL;DR: Length–weight relationships are given on six freshwater fish species from four streams flowing into Lake Sapanca, north-western Turkey.
Abstract: Summary
Length–weight relationships are given on six freshwater fish species (Phoxinus phoxinus, Cobitis vardarensis, Neogobius gymnotrachelus, Proterorhinus marmoratus, Petroleuciscus borysthenicus and Gambusia holbrooki) from four streams flowing into Lake Sapanca, north-western Turkey.