Chromatography software

Abstract: Part and chapter headings: Individual Chromatographic Modes. Ion exchange chromatography (P.H. Roos). Size exclusion chromatography (M. Roegner). Reversed-phase chromatography (H. Schluter). Hydrophobic interaction chromatography (L.R. Jacob). Hydroxyapatite chromatography (R. Luka in, W.R. Deppert). Immobilized metal ion affinity chromatography (M. Kastner). Chromatofocusing (R. Luka in, W.R. Deppert). Dye-ligand affinity chromatography (J. Kirchberger, H.-J. Boehme). Immobilized artificial membrane chromatography (K. Hauer et al.). Liquid-liquid partition chromatography (U.-B. Hansson, C. Wingren). Displacement chromatography (H. Schluter, J. Jankowski). Various Target Protein Classes. Integral membrane proteins (G. Welling, S. Welling-Wester). Recombinant proteins (O. Huber, M. Huber-Wunderlich). DNA-binding proteins (M. Velleman). Lectins and glycoconjugates (H.-J. Gabius). Covalent chromatography (D. Whitney). Cell affinity chromatography (D.D. Putnam et al.). Miscellaneous Chromatographic Aspects. Coupling reactions (E. Muller). Immobilization of nucleotides (E. Muller, M. Morr). Miscellaneous biospecific affinity gels (A.M. Williams). Scale-up of downstream processing (J. Walter). Chromatography on porous matrices (J. Kruip). Chromatography using Strep-tag affinity peptides (H. N. Muller, T.G.M. Schmidt). Buffers and additives (W.R. Deppert, R. Luka in). Chromatography software (T. Steger-Hartmann). Supplement. Abbreviations used in this book. Directory of manufacturers and suppliers. Subject index.

Abstract: A theoretical study of the effect of stationary phase parameters on the regularities of the separation of peptides and proteins using a model of critical chromatography BioLCCC and the Theoretical chromatography software created on its basis has been performed. The following problems have been discussed: mechanism of the migration of chromatographic peak along the column and its compression in gradient elution; dependence of the coefficient of protein separation on the column length; inversion of the elution order of peptides at a change of the diameter or length of the column, adsorbent nature, conditions of emergence of inversion; and anomaly of the dependence of the retention time of proteins on the adsorbent activity.

Abstract: The invention discloses a lock-in thermal imaging chromatography representation system and method of an integrated circuit having microdefect. The lock-in thermal imaging chromatography representationsystem includes a medium wave infrared camera, a data collection card, a computer, a three dimensional mobile table and a DC power supply, wherein the computer controls the data collection card to control the DC power supply to trigger and performs amplitude modulation changing on an integrated circuit sample piece, so as to enable the current with constant frequency to inject; at the same time,the computer controls the data collection card to control the medium wave infrared camera to synchronously trigger collected image sequences; the image sequences collected by the medium wave infraredcamera are transmitted to the computer to perform synchronous lock-in processing, so as to obtain an amplitude and phase diagram of different frequency by changing the frequency; and lock-in thermal imaging chromatography software of the computer is used to obtain the deep chromatography result of the integrated circuit sample piece. The lock-in thermal imaging chromatography representation systemand method of an integrated circuit having microdefect is an infrared thermal wave nondestructive test new method having the advantages of high signal to noise ratio, nondestructiveness, quickness, visuality, accuracy, large detection area, high efficiency and the like.