Choanoderm

Abstract: The calcareous sponge Leucosolenia laxa releases free-swimming hollow larvae called coeloblastulae that are the characteristic larvae of the subclass Calcinea. Although the coeloblastula is a major type of sponge larva, our knowledge about its development is scanty. Detailed electron microscopic studies on the metamorphosis of the coeloblastula revealed that the larva consists of four types of cells: flagellated cells, bottle cells, vesicular cells, and free cells in a central cavity. The flagellated cells, the principal cell type of the larva, are arranged in a pseudostratified layer around a large central cavity. The larval flagellated cells characteristically have glutinous granules that are used as internal markers during metamorphosis. After a free-swimming period the larva settles on the substratum, and settlement apparently triggers the initiation of metamorphosis. The larval flagellated cells soon lose their flagellum and begin the process of dedifferentiation. Then the larva becomes a mass of dedifferentiated cells in which many autophagosomes are found. Within 18 h after settlement, the cells at the surface of the cell mass differentiate to pinacocytes. The cells beneath the pinacoderm differentiate to scleroblasts that form triradiate spicules. Finally, the cells of the inner cell mass differentiate to choanocytes and are arranged in a choanoderm that surrounds a newly formed large gastral cavity. We found glutinous granules in these three principal cell types of juvenile sponges, thus indicating the multipotency of the flagellated cells of the coeloblastula.

Abstract: Sponges (Porifera) demonstrate prominent regeneration abilities and possess a wide variety of mechanisms, used during this process. In the current study, we combined in vivo observations with histological, immunohistochemical, and ultrastructural technics to elucidate the fine cellular mechanisms of the regeneration in the calcareous sponge Leucosolenia cf. variabilis. The regeneration of Leucosolenia cf. variabilis ends within 4-6 days. The crucial step of the process is the formation of the transient regenerative membrane, formed by the epithelial morphogenesis-spreading of the intact exopinacoderm and choanoderm. The spreading of the choanoderm is accompanied by the transdifferentiation of the choanocytes. The regenerative membrane develops without any contribution of the mesohyl cells. Subsequently, the membrane gradually transforms into the body wall. The cell proliferation is neither affected nor contributes to the regeneration at any stage. Thus, Leucosolenia cf. variabilis regeneration relies on the remodeling of the intact tissues through the epithelial morphogenesis, accompanied by the transdifferentiation of some differentiated cell types, which makes it similar to the regeneration in homoscleromorphs and eumetazoans.

Abstract: The free-swimming parenchymella larvae of Haliclona permollis have a surface of flagellated cells that function in locomotion. These flagellated cells disappear from the larval surface soon after larval settlement, but the debate about their fate during metamorphosis has not been resolved. An improved method for electron microscopy enabled us to visualize minute ellipsoid granules characteristic of the larval flagellated cells of this sponge and to use these granules as natural markers to follow the fate of flagellated cells in metamorphs. On metamorphosis, the axonemes of the flagellated cells are withdrawn into the cell body and persist for some time, thus serving as a second natural marker for flagellated cells in postlarval forms. Within 12 h after settlement, the metamorphs have both markers in amoeboid cells derived from flagellated cells. The minute ellipsoid granules are found in the amoeboid cells of the metamorphs 24 h after settlement, and in the choanocytes of the juveniles 36 h after settlement. Therefore, it is proposed that the choanocytes derive from the larval flagellated cells by way of an amoeboid cell stage. These results suggest that the flagellated cells of parenchymella larvae participate in the formation of juveniles.

Abstract: Homosclerophorida Dendy (Demospongiae, Homoscleromorpha) contains a single family Plakinidae Schulze (including Oscarellidae Lendenfeld and Corticiidae Vosmaer), with seven valid genera and about 60 valid species worldwide. Species live mainly in shallow waters but a few have been recorded from abyssal depths (up to 2460m). Species are often encrusting, lobate, but massive species are common in some genera (Plakortis, Plakinastrella); surface is usually smooth or microhispid and consistency varies from soft to cartilaginous. All genera possess flagellated exo- and endopinacocytes, a basement membrane lining both choanoderm and pinacoderm, oval to spherical choanocyte chambers with a sylleibid-like or leuconoid organization, and a unique incubated cinctoblastula-type larvae; spicules, when present, are peculiar tetractines (calthrops) and derivatives. Genera are distinguished mainly by four morphological characters: presence of a siliceous skeleton; presence of a cortex associated with a leuconoid aquiferous system and well-developed mesohyl or a sylleibid aquiferous system with poorly developed mesohyl and ectosome; number of spicule size classes; and presence and type of ramifications in the actines of calthrops (tetractinal spicules), with three distinct general morphologies recognized.