Topic
Chloroacetamide
About: Chloroacetamide is a research topic. Over the lifetime, 322 publications have been published within this topic receiving 3287 citations. The topic is also known as: 2-chloroacetamide.
Papers published on a yearly basis
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Papers
TL;DR: The sequence information for the isolated GST, which is designated “GST IV” indicates that the enzyme is a unique maize GST but shares some homology with maize GSTs I and III.
Abstract: A glutathione S-transferase (GST) isozyme from maize (Zea mays Pioneer hybrid 3906) treated with the dichloroacetamide herbicide safener benoxacor (CGA-154281) was purified to homogeneity and partially characterized. The enzyme, assayed with metolachlor as a substrate, was purified approximately 200-fold by ammonium sulfate precipitation, anion-exchange chromatography on Mono Q resins, and affinity chromatography on S-hexylglutathione agarose from total GST activity present in etiolated shoots. The purified protein migrated during sodium dodecyl sulfate-polyacrylamide gel electrophoresis (PAGE) as a single band with a molecular mass of 27 kD. Using nondenaturing PAGE, we determined that the native protein has a molecular mass of about 57 kD and that the protein exists as a dimer. Two-dimensional electrophoresis revealed only a single protein with an isoelectric point of 5.75 and molecular mass of 27 kD. These results further suggest that the protein exists as a homodimer of two identical 27-kD subunits. The enzyme was most active with substrates possessing a chloroacetamide structure. trans-Cinnamic acid and 1-chloro-2,4-dinitrobenzene were not effective substrates. Apparent Km values for the enzyme were 10.8 microM for the chloroacetamide metolachlor and 292 microM for glutathione. The enzyme was active from pH 6 to 9, with a pH optimum between 7.5 and 8. An apparently blocked amino terminus of the intact protein prevented direct amino acid sequencing. The enzyme was digested with trypsin, and the amino acid sequences of several peptide fragments were obtained. The sequence information for the isolated GST we have designated "GST IV" indicates that the enzyme is a unique maize GST but shares some homology with maize GSTs I and III.
129 citations
TL;DR: This study highlights an important potential use of strain FLY-8 for the in situ bioremediation of chloroacetamide herbicides and their metabolite-contaminated environment.
Abstract: A butachlor-degrading strain, designated FLY-8, was isolated from rice field soil and was identified as Paracoccus sp. Strain FLY-8 could degrade and utilize six chloroacetamide herbicides as carbo...
117 citations
TL;DR: A survey of source waters used by 12 drinking water utilities in the Midwestern United States found groundwater sources tended to have lower concentrations of parents and neutral degradates than surface water sources in the fall, although concentrations in groundwater were similar to those in surface water in the spring.
84 citations
TL;DR: Solid-phase microextraction coupled with gas chromatography-mass spectrometry was used to analyze two triazine and simazine and three chloroacetamide herbicides in water samples from a midwest US agricultural drainage ditch, indicating that atrazine levels in the ditch water exceeded the US maximum contaminant level for drinking water 12% of the time.
79 citations
TL;DR: A new methodology is described which allows easy access to water-soluble, biodegradable glycopolymers with both predeterminable composition and molecular weight distribution.
Abstract: Glycopolymers are useful macromolecules with a non-carbohydrate backbone for presenting saccharides in a multivalent form Here, a new methodology is described which allows easy access to water-soluble, biodegradable glycopolymers with both predeterminable composition and molecular weight distribution Thus, chloroacetylation of commercially available polylysine hydrobromide 3 gave the reactive homopolymer 4, whose chloroacetamide functions allowed subsequent coupling with thiol-containing components Water-soluble homopolymers such as 8 and 13 were available by treatment with an excess of hydrophilic thiols Heteroglycopolymers were obtained via quantitative incorporation of substoichiometric amounts of carbohydrates with a mercapto functionality linked to the reducing end; the remaining chloroacetamide groups were capped with an excess of thioglycerol A variety of glycopolymers with up to four different components was prepared The composition and purity of the products were reliably analyzed by 1H NMR
78 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 9 |
| 2020 | 10 |
| 2019 | 8 |
| 2018 | 2 |
| 2017 | 11 |
| 2016 | 8 |