TL;DR: The application of the “top-to-bottom approach” using 16S rRNA probes and in situ hybridization to assess the microbial diversity in Aplysina sponges is described for the first time.
Abstract: We have employed electronmicroscopical methods (SEM, TEM) to document the microbial community associated with the marine sponge Aplysina cavernicola (formerly Verongia cavernicola, class Demospongiae). Five dominant bacterial types were identified, three of which resemble the morphotypes originally described by Vacelet (1975). One bacterial type possesses morphological properties that are characteristic of the genus Planctomyces. In addition, morphologically uniform bacteria which reside inside the nuclei of host cells were observed. Using in situ hybridization with fluorescently labelled rRNA probes directed against known bacterial groups, the phylogenetic affiliation of the mesohyl bacteria was assessed. It could be shown that the vast majority of mesohyl bacteria belongs to the domain Bacteria with a low GC content. Among the Bacteria, the delta-Proteobacteria were most abundant, followed by the gamma-Proteobacteria and representatives of the Bacteroides cluster. Clusters of Gram-positive bacteria with a high GC content were also found consistently in low amounts. No hybridization signal was obtained with probes specific to the domain Archaea, to the alpha- and beta-Proteobacteria and to the Cytophaga/Flavobacterium cluster. This study describes for the first time the application of the “top-to-bottom approach” using 16S rRNA probes and in situ hybridization to assess the microbial diversity in Aplysina sponges.
TL;DR: The dark, mostly brackish, aquatic habitats along the coasts are inhabited by a number of phylogenetically and/or biogeographically interesting animal taxa, whose ecology is poorly known.
Abstract: The dark, mostly brackish, aquatic habitats along the coasts are inhabited by a number of phylogenetically and/or biogeographically interesting animal taxa, whose ecology is poorly known. We are especially ignorant about the nature of the deoxygenated strata that occur in most of these habitats. Are these strata anoxic or only dysoxic? And how are their inhabitants adapted to low oxygen tensions?
TL;DR: A new genus and species of terrestrial cavernicolan triclad flatworm from Barro Colorado Island, Panama is provided, with the following characteristics: absence of pigmentation and eyes.
Abstract: The paper provides a taxonomic description of a new genus and species of terrestrial cavernicolan triclad flatworm from Barro Colorado Island, Panama. Molecular sequences of this species were already deposited in GenBank several years ago under the provisional taxon name Cavernicola sp. Animals have the following characteristics: absence of pigmentation and eyes; mouth opening located halfway in the pharyngeal cavity; few, ventral testes extending from a position far posterior to the ovaries to directly behind the pharyngeal cavity; strongly recurving sperm ducts, thus forming a loop; horizontally oriented cone-shaped penis papilla; common oviduct oriented perpendicular to the bursal canal and opening into the latter shortly before the canal communicates with the copulatory bursa; sac-shaped copulatory bursa lacking a distinct, single lumen and filled with a mass of syncytial cells, with interspersed nuclei.
TL;DR: Twenty-seven species of terrestrial Isopoda are recorded from Socotra Island (Yemen) and three species are described as new, highlighting the high number of endemics present on this archipelago.
Abstract: Twenty-seven species of terrestrial Isopoda are recorded from Socotra Island (Yemen) One genus (Dumetoniscus) in the family Bathytropidae and three species (Serendibia cavernicola, Dumetoniscus graniticus and Platyarthrus alticolus) are described as new A key to the 41 species of Oniscidea presently known from the Socotra Archipelago is given Th e new records confi rm the high number of endemics present on this archipelago
TL;DR: All the seven species now in Setosella have present-day representatives; the geographic distribution of the genus is restricted to the NE Atlantic and adjacent seas; its stratigrafic distribution is rejuvenated to the late Tortonian-early Messinian of southern Italy and insular Greece, with only three species occasionally and discontinuously reported.
Abstract: The genus Setosella included to date six species. After revision, only four of these species were retained, i.e. S. vulnerata, S. cavernicola, S. folini and S. spiralis. The remaining two species were tentatively placed in Woodipora, W.? antilleana n. comb., and Andreella, A.? fragilis n. comb. On the other hand, scanning electron microscopy examination of Mediterranean material, revealed the presence of three new species previously included in species complexes: S. cyclopensis n. sp. from the open-shelf, S. rossanae n. sp. from submarine caves, and S. alfioi n. sp. from deep-waters. The diagnosis of the genus was amended to include the occurrence of kenozooids in S. cyclopensis n. sp. and S. cavernicola, and of free-living ring-shaped and scorpioid colonies in S. folini and S. alfioi n. sp. All the seven species now in Setosella have present-day representatives; the geographic distribution of the genus is restricted to the NE Atlantic and adjacent seas; its stratigrafic distribution is rejuvenated to the late Tortonian-early Messinian of southern Italy and insular Greece, with only three species occasionally and discontinuously reported. All species are able to produce numerous, subsequent intramural buds and morphological differences seem to be consistently associated with exploitation of particular habitats and substrata.