TL;DR: High frecuency axillary branching was induced in shoots of Cattleya mossiae on Huangs modification of Murashige and Skoogs medium containing 1 mg/L of benzyladenine and thidiazuron and no differences were detected by the use of polypropylene membranes or two levels of MS inorganic salts on root proliferation.
Abstract: Micropropagation of Cattleya mossiae Parker ex Hook through thidiazuron-induced axillary branching High frecuency axillary branching was induced in shoots of Cattleya mossiae on Huangs modification of Murashige and Skoogs medium containing 1 mg/L of benzyladenine (BA) and 1.0 or 3.3 µM/L of thidiazuron (TDZ). Cumulative and residual effects of TDZ were detected. The first consisted in an increase of axillary branching and protocorm-like bodies (PLB) proliferation, and the second consisted in an increase of PLB and fasciated shoot formation. Concentrations of TDZ above 1 µM/L had an inhibitory effect on shoot elongation. No differences were detected by the use of polypropylene membranes or two levels of MS inorganic salts on root proliferation.
TL;DR: To score seed quality, seeds from nine Cattleya species were germinated on Knudson C medium and viability was measured using a tetrazolium test following pre-conditioning in a 10% sucrose solution, both before and after storage at −18°C for 3 mo.
Abstract: Effective seed storage methods for orchid species provide a unique opportunity to preserve large amounts of germplasm. Early evaluation of the physiological status of seeds is useful for selecting seed lots for conservation. To score seed quality, seeds from nine Cattleya species (Cattleya granulosa, Cattleya hegeriana, Cattleya intermedia, Cattleya mossiae, Cattleya purpurata, Cattleya sanguiloba, Cattleya tenuis, Cattleya tigrina and Cattleya walkeriana) were germinated on Knudson C medium and viability was measured using a tetrazolium (TZ) test following pre-conditioning in a 10% sucrose solution, both before and after storage at −18°C for 3 mo. Tetrazolium test results were quantified using digital imaging of germinating seeds on a flatbed scanner. Seed viability (with or without pre-conditioning in sucrose), germination, Germination Velocity Index (GVI) and the correlation between viability measurements and germination were also evaluated. Both the tetrazolium test and the germination results showed that there was no loss of viability after storage, although in five seed lots, the GVI decreased. Pre-conditioning improved the correlation between viability exhibited in the tetrazolium test and seed germination. Image analysis proved to be a powerful tool for analysing these small seeds.