Carboy

Abstract: A convenient method is described for the production of up to 1.75 g of citrinin per liter by Penicillium citrinum growing in stationary culture in a 5-gallon (18.925 liters) carboy containing 4 liters of 4% sucrose and 2% yeast extract medium.

Abstract: A bioreactor system for biotoxin production was appraised against traditional methods of growing dinoflagellate cultures. In an optimised bioreactor culture (5.4 L) operated in batch mode, growth of Karenia selliformis was more efficient than in 15-L bulk carboy culture in terms of growth rate (μ = 0.07 day−1 versus 0.05 day−1) and growth maximum (G max, 169.106 versus 41.106 cells L−1). Maximal gymnodimine concentration (1200 μg L−1) in bioreactor culture was 8-fold higher than in bulk carboy culture, and the yield per cell (pg cell−1) was 2-fold higher. Similarly the bioreactor batch culture of Alexandrium ostenfeldii performed more efficiently than carboy cultures in terms of growth rate (1.6-fold higher), growth maximum (15-fold higher) and desmethyl C spirolide (SPX-desMe-C) yield (5-fold higher [μg L−1], though the yield [pg cell−1basis] was lower). When bioreactor cultures of K. selliformis were operated in continuous mode, the yield of gymnodimine was substantially higher than a carboy or the bioreactor run in batch mode to growth max (793 μg day−1 over 58 days in continuous culture was achieved versus an average of 60 μg day−1 [carboy over 40 days] or 249 μg day−1 [batch mode] over 26 days). Likewise in continuous bioreactor cultures of A. ostenfeldii run over 25 days, the yield of SPX-desMe-C (29 μg day−1) was substantially higher than in same cultures run in batch mode or carboys (10.2 day−1 and 7.7 μg day−1 respectively). Similarly 5.4 L bioreactor batch cultures of K. brevisulcata reached 3.8-fold higher cell densities than carboy cultures, and when operated in continuous mode, the brevisulcatic acids were more efficiently produced than in batch culture (12 μg day−1 versus 7 μg day−1). When the bioreactor system was upscaled to 52 L, the maximum cell densities and toxin yields of K. brevisulcata cultures were somewhat less than those achieved in the smaller reactor, which was attributed to reduced light penetration.

Abstract: An apparatus for the continuous production of high-purity water includes a distillation flask, a carboy and a condenser unit, all of borosilicate glass. A respective filter is provided between the ambient atmosphere and the interiors of the flask, the carboy and the condenser unit to remove airborne bacteria and dust particles. An inlet valve is provided for feeding water to be purified into the flask, all water-contacting parts of this valve being of polytetrafluoroethylene. The open parts are interconnected with flexible, polytetrafluoroethylene tubing. A two-way, stopcock allows high-purity water to be removed from the carboy.

Abstract: An apparatus for the continuous production of high-purity water includes a distillation flask, a carboy and a condenser unit, all of borosilicate glass. A respective filter, preferably in the form of a polytetrafluoroethylene sheet, is provided between the ambient atmosphere and the interiors of the flask, the carboy and the condenser unit to remove airborne bacteria and dust particles. An inlet valve is provided for feeding water to be purified into the flask, all water-contacting parts of this valve being of polytetrafluoroethylene. The open parts are interconnected with flexible, polytetrafluoroethylene tubing. A two-way, stopcock allows high-purity water to be removed from the carboy. The flask is provided with a glass overflow trap. In one variant, the water is fed into the flask via members between the trap and the flask. In a preferred variant, the water is fed into the flask via a separate inlet and allowed to flow out through the overflow trap, thereby allowing precipitate to be constantly washed out of the flask.