Topic
Calcein
About: Calcein is a research topic. Over the lifetime, 993 publications have been published within this topic receiving 38712 citations. The topic is also known as: N,N'-(3',6'-Dihydroxyspiro(phthalid-3,9'-xanthen)-2',7'-diyldimethyl)bis(iminodiessigsaeure) & 2,7-Bis(N,N-bis(carboxymethyl)aminomethylene)fluorescein.
Papers published on a yearly basis
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Papers
Journal Article•
TL;DR: Measurements of radiation-induced mitogen-activated protein kinase activity indicate that this sensing/amplification mechanism is necessary for activation of some cytoplasmic signaling pathways by low doses of radiation.
Abstract: Transient generation of reactive oxygen or nitrogen (ROS/RNS), detected with dihydrodichlorofluoroscein by fluorescence microscopy, occurs within minutes of exposing cells to ionizing radiation. In the 1-10 Gy dose range, the amount of ROS/RNS produced/cell is constant, but the percentage of producing cells increases with dose (20 to 80%). Reversible depolarization of the mitochondrial membrane potential () and decrease in fluorescence of a mitochondria-entrapped dye, calcein, are observed coincidentally. Radiation-induced ROS/RNS, depolarization, and calcein fluorescence decrease are inhibited by the mitochondrial permeability transition inhibitor, cyclosporin A, but not the structural analogue, cyclosporin H. Radiation-stimulated ROS/RNS is also inhibited by overexpressing the Ca(2+)-binding protein, calbindin 28K, or treating cells with an intracellular Ca(2+) chelator. Radiation-induced ROS/RNS is observed in several cell types with the exception of rho(o) cells deficient in mitochondrial electron transport. rho(o) cells show neither radiation-induced ROS/RNS production nor depolarization. We propose that radiation damage in a few mitochondria is transmitted via a reversible, Ca(2+)-dependent mitochondrial permeability transition to adjacent mitochondria with resulting enhanced ROS/RNS generation. Measurements of radiation-induced mitogen-activated protein kinase activity indicate that this sensing/amplification mechanism is necessary for activation of some cytoplasmic signaling pathways by low doses of radiation.
524 citations
TL;DR: The intracellular covalent coupling dye carboxyfluorescein diacetate succinimidyl ester (CFSE) appears to have the edge over PKH26 based on homogeneity of lymphocyte staining and cost, while two other fluorescent dyes, although not suitable for lymphocyte proliferation studies, are valuable tracking dyes for short‐term lymphocyte migration experiments.
Abstract: Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the most versatile in terms of long-term tracking of lymphocytes and their ability to quantify lymphocyte proliferation. They are the intracellular covalent coupling dye carboxyfluorescein diacetate succinimidyl ester (CFSE) and the membrane inserting dye PKH26. Both dyes have the advantage that they can be used to track cell division, both in vitro and in vivo, due to the progressive halving of the fluorescence intensity of the dyes in cells after each division. However, CFSE appears to have the edge over PKH26 based on homogeneity of lymphocyte staining and cost. Two other fluorescent dyes, although not suitable for lymphocyte proliferation studies, are valuable tracking dyes for short-term (up to 3 day) lymphocyte migration experiments, namely the DNA-binding dye Hoechst 33342 and the cytoplasmic dye calcein. In the future it is highly likely that additional fluorescent dyes, with different spectral properties to CFSE, will become available, as well as membrane inserting fluorescent dyes that more homogeneously label lymphocytes than PKH26.
523 citations
TL;DR: Liposome fusion on mesoporous particles are reported as a synergistic means to simultaneously load and seal cargo within the porous core of a porous core.
Abstract: Mixing liposomes with hydrophilic particles induces fusion of the liposome onto the particle surface. Such supported bilayers have been studied extensively as models of the cell membrane, while their applications in drug delivery have not been pursued. In this communication, we report liposome fusion on mesoporous particles as a synergistic means to simultaneously load and seal cargo within the porous core. We find fusion of a cationic lipid (DOTAP) on an anionic silica particle loads an anionic fluorescent dye (calcein) into the particle to a concentration exceeding 100× that in the surrounding medium. The loaded “protocell” particles are taken up efficiently by Chinese hamster ovary cells, where, due to a reduced pH within endosomal compartments, calcein is effectively released. Compared to some other nanoparticle systems, protocells provide a simple construct for cargo loading, sealing, delivery, and release. They promise to serve as useful vectors in nanomedicine.
377 citations
TL;DR: Calcein encapsulation in the Zr-based MOF UiO-66 is reported, followed by amorphization of the framework by ball-milling, and controlled release of calcein over more than 30 days is shown.
364 citations
TL;DR: These studies establish a calcein quenching method for measurement of cell membrane water permeability and indicate that AQP-4 provides the principal route for water transport in astrocytes.
Abstract: A calcein fluorescence quenching method was applied to measure osmotic water permeability in highly differentiated primary cultures of brain astrocytes from wild-type and aquaporin-4 (AQP-4)-defici...
353 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2025 | 9 |
| 2024 | 26 |
| 2023 | 64 |
| 2022 | 99 |
| 2021 | 15 |
| 2020 | 37 |