TL;DR: Shadowing, elevation, and storage significantly affected the transcription of the allergen-encoding genes, whereas water stress slightly influenced the expression of only two genes, in spite of the dramatic effect on both fruit size and vegetative growth of the trees.
Abstract: Freshly consumed apples can cause allergic reactions because of the presence of four classes of allergens, namely, Mal d 1, Mal d 2, Mal d 3, and Mal d 4, and their cross-reactivity with sensitizing allergens of other species. Knowledge of environmental and endogenous factors affecting the allergenic potential of apples would provide important information to apple breeders, growers, and consumers for the selection of hypoallergenic genotypes, the adoption of agronomical practices decreasing the allergenic potential, and the consumption of fruits with reduced amount of allergens. In the present research, expression studies were performed by means of real-time PCR for all the known allergen-encoding genes in apple. Fruit samples were collected from 15 apple varieties and from fruits of three different trials, set up to assess the effect of shadowing, elevation, storage, and water stress on the expression of allergen genes. Principal components analysis (PCA) was performed for the classification of varieties according to gene expression values, pointing out that the cultivars Fuji and Brina were two good hypoallergenic candidates. Shadowing, elevation, and storage significantly affected the transcription of the allergen-encoding genes, whereas water stress slightly influenced the expression of only two genes, in spite of the dramatic effect on both fruit size and vegetative growth of the trees. In particular, shadowing may represent an important cultural practice aimed at reducing apple cortex allergenicity. Moreover, elevation and storage may be combined to reduce the allergenic potential of apple fruits. The possible implications of the results for breeders, growers, and consumers are discussed critically.
TL;DR: The eleven cultivars common to both localities are compared as regards the quality of fruits grown in different environments and the eleven new cultivars not yet commercially available are discussed.
Abstract: Two experimental fields have been planted in Lombardia with scab resistant apple cultivars, one in the plane, in the Po valley (Lodi province) at 85 m above sea level, the other in Valtellina at 400 m above sea level. Eleven cultivars (Brina, Baujade, Enterprise, Golden Lasa, Golden Mira, Golden Orange, Gold Rush, Nova, Primiera, Sir Prize and Topaz) are present in both localities, five more (Criterion, Harmony Delorina, Red Early, Summerfree and Sansa) are present only at Arcagna, while seven more are present only in Valtellina (Florina, Freedom, Prima, Rajka, Rubinola, Rosana, Otava). In the Valtellina orchard there are also thirteen new cultivars not yet commercially available (CLR13T40, CLR13T45, CLR20T14, CLR20T22, CQR10T17, HER4T175, Pri Coop 11, Pri Coop 17, Pri Coop 25, Pri Coop 34, Pri 2463/101, Pri 75 441/67, PSER11T27). In both localities observations were made on the agronomic behaviour of trees (vigour and branching of shoots, susceptibility to scab or to other pathologies of leaves or fruits, fruit set, fruit bearing, etc.); phenological phases (time of budding, flowering, ripening of fruits) were recorded; fruit morphological characteristics (weight, shape, colour, skin russeting etc.) were observed at harvest. Fruits were moreover analyzed at harvest and after storage for chemical and physical characteristics. Observations on performance of cultivars and fruit quality are shown and discussed. The eleven cultivars common to both localities are compared as regards the quality of fruits grown in different environments.
TL;DR: Among the tested cultivars or selections most of asymptomatic trees of cultivars Brina and Enova resulted to be positive to phytoplasma presence, while in selection CO-OP12 only one out of 34 tested plants resulted positive to AP infection.
Abstract: Severe apple proliferation (AP) epidemics are spread in the most important apple growing areas in Europe with a significant economic impact on the production of marketable fruits. A genotype screening was undertaken on 41 apple cultivars and selections showing no phytoplasma-related symptoms to identify natural resistance or tolerance to ‘Candidatus Phytoplasma mali’. Among the tested cultivars or selections most of asymptomatic trees of cultivars Brina and Enova resulted to be positive to phytoplasma presence, while in selection CO-OP12 only one out of 34 tested plants resulted positive to AP infection.