Topic
Bhargavaea
About: Bhargavaea is a research topic. Over the lifetime, 3 publications have been published within this topic receiving 25 citations.
Papers
TL;DR: Phylogenetic analyses using neighbour-joining, minimum-evolution and maximum-parsimony methods indicated that strain DSE10(T) formed a deeply rooted lineage distinct from the clades represented by the genera Planococcus, Planomicrobium, Bacillus and Geobacillus.
Abstract: A novel Gram-positive, rod-shaped, non-motile, non-spore-forming bacterium, strain DSE10T, was isolated from a deep-sea sediment sample collected at a depth of 5904 m from the Chagos–Laccadive ridge system in the Indian Ocean. Cells of strain DSE10T were positive for catalase, oxidase, urease and lipase activities and contained iso-C14 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0 as the major fatty acids. The major respiratory quinones were MK-6 and MK-8 and the major lipids were phosphatidylglycerol and diphosphatidylglycerol. The cell-wall peptidoglycan contained diaminopimelic acid as the diagnostic diamino acid. A blast sequence similarity search based on 16S rRNA gene sequences indicated that the genera Planococcus, Planomicrobium, Bacillus and Geobacillus were the nearest phylogenetic neighbours to the novel isolate with gene sequence similarities ranging from 94.9 to 95.2 %. Phylogenetic analyses using neighbour-joining, minimum-evolution and maximum-parsimony methods indicated that strain DSE10T formed a deeply rooted lineage distinct from the clades represented by the genera Planococcus, Planomicrobium, Bacillus and Geobacillus. Further, strain DSE10T could be distinguished from the above-mentioned genera based on the presence of signature nucleotides G, A, C, T, C, A, G, C and T at positions 182, 444, 480, 492, 563, 931, 1253, 1300 and 1391, respectively, in the 16S rRNA gene sequence. Based on the phenotypic and phylogenetic characteristics determined in this study, strain DSE10T was assigned as the type species of a new genus, Bhargavaea gen. nov., as Bhargavaea
cecembensis sp. nov. The type strain of Bhargavaea cecembensis gen. nov., sp. nov. is DSE10T (=LMG 24411T=JCM 14375T). The genomic DNA G+C content of strain DSE10T is 59.5±2.5 mol%.
26 citations
TL;DR: Phylogenetic analysis indicated that strain S1203T formed a lineage within the family Bacillaceae that was distinct from the most closely related genera Bacillus, Bhargavaea, Planomicrobium and Virgibacillus with gene sequence similarities ranging from 86.2 to 93.76 %.
Abstract: A Gram-staining-positive, strictly aerobic, spore-forming and rod-shaped motile bacterium with peritrichous flagellae, designated strain S1203T, was isolated from the sediment of the northern Okinawa Trough. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S1203T formed a lineage within the family Bacillaceae that was distinct from the most closely related genera Bacillus, Bhargavaea, Planomicrobium and Virgibacillus with gene sequence similarities ranging from 86.2 to 93.76 %. Optimal growth occurred in the presence of 4-8 % (w/v) NaCl, at pH 7.0-8.0 and 25-32 °C. The cell-wall peptidoglycan was based on meso-diaminopimelic acid and unsaturated menaquinone with seven isoprene units (MK-7) as the predominant respiratory quinone. The major fatty acids (>10 % of total fatty acids) were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. The DNA G+C content of strain S1203T was 47.7 mol%. On the basis of polyphasic analysis, strainS1203T was considered to represent a novel species in a new genus of the family Bacillaceae, for which the name Aureibacillus halotolerans gen. nov., sp. nov. is proposed; the type strain of Aureibacillus halotolerans is S1203T ( = DSM 28697T = JCM 30067T = MCCC 1K00259T).
6 citations
TL;DR: On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain 201802YP6T represents a novel species of the genus Bhargavaea, for which the name Bhargvaea changchunensis sp.
Abstract: A Gram-staining-positive, aerobic, rod-shaped (201802YP6T) bacteria was isolated from soil, Northeast of China Growth occurred at 10–40 °C (optimum 25–30 °C), at pH 60–80 (optimum 70) and at 0–2% NaCl Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbors of strain 201802YP6T were identified as Bhargavaea cecembensis DSE10T (9952%), Bhargavaea beijingensis ge10T (9945%), Bhargavaea indica KJW98T (9945%), Bhargavaea ullalensis ZMA19T (9881%), and Bhargavaea ginsengi ge14T (9876%) Levels of similarity among strain 201802YP6T and other Bhargavaea species were lower than 9876% GyrB amino acid sequence-based analysis supported the phylogenetic position and also distinguished strain 201802YP6T from the other species of the genus Bhargavaea DNA–DNA hybridization values between strain 201802YP6T and B cecembensis, B beijingensis, B indica, B ullalensis, B ginsengi were 435%, 43%, 325%, 305% and 204%, respectively The DNA G + C content of strain 201802YP6T was 5123 mol% The average nucleotide identity (ANI) of the draft genome was 8704% to B cecembensis DSE10T The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipids, phosphatidylethanolamine, and phosphatidyllipid The predominant menaquinone was MK-8 The major fatty acids were iso-C15:0 (3991%), anteiso-C15:0 (2886%), anteiso-C17:0 (630%) and C16:0 (613%) On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain 201802YP6T represents a novel species of the genus Bhargavaea, for which the name Bhargavaea changchunensis sp nov is proposed The type strain is 201802YP6T (= CGMCC 113508T = KCTC 33975T)
2 citations
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| Year | Papers |
|---|---|
| 2018 | 1 |
| 2015 | 1 |
| 2009 | 1 |