TL;DR: Although the amount of allelic variation is low when compared with terrestrial mammals, the nature of the substitutions in the peptide binding sites indicates an important role for the DQ beta locus in the cellular immune response of beluga whales.
Abstract: Genetic variation at the Major Histocompatibility Complex locus DQ beta was analyzed in 233 beluga whales (Delphinapterus leucas) from seven populations: St. Lawrence Estuary, eastern Beaufort Sea, eastern Chukchi Sea, western Hudson Bay, eastern Hudson Bay, southeastern Baffin Island, and High Arctic and in 12 narwhals (Monodon monoceros) sympatric with the High Arctic beluga population. Variation was assessed by amplification of the exon coding for the peptide binding region via the polymerase chain reaction, followed by either cloning and DNA sequencing or single-stranded conformation polymorphism analysis. Five alleles were found across the beluga populations and one in the narwhal. Pairwise comparisons of these alleles showed a 5:1 ratio of nonsynonymous to synonymous substitutions per site leading to eight amino acid differences, five of which were nonconservative substitutions, centered around positions previously shown to be important for peptide binding. Although the amount of allelic variation is low when compared with terrestrial mammals, the nature of the substitutions in the peptide binding sites indicates an important role for the DQ beta locus in the cellular immune response of beluga whales. Comparisons of allele frequencies among populations show the High Arctic population to be different (P < or = .005) from the other beluga populations surveyed. In these other populations an allele, Dele-DQ beta*0101-2, was found in 98% of the animals, while in the High Arctic it was found in only 52% of the animals. Two other alleles were found at high frequencies in the High Arctic population, one being very similar to the single allele found in narwhal.
TL;DR: In this article, an analysis of over 2000 bones recovered from a semisubterranean house at Gupuk, a Mackenzie Inuit archaeological site on the East Channel of the Mackenzie River is presented.
Abstract: Ethnohistoric records indicate that the economy of early historic Mackenzie Inuit was centred on the summer hunt for beluga whales ( Delphinapterus leucas ). However, no systematic attempt has been made to quantify the dietary importance of beluga whales to earlier, precontact-period Mackenzie Inuit societies. This issue is addressed herein through analysis of over 2000 beluga bones recovered from a semisubterranean house at Gupuk, a Mackenzie Inuit archaeological site on the East Channel of the Mackenzie River. The amount of meat and fat available from beluga whales is compared to that from all other prey species at the site to assess the relative dietary contribution of each taxon. The results indicate that beluga whales were a truly focal resource in the local economy, probably providing over half of the food available to residents of Gupuk and other communities in the Mackenzie Delta for at least half of each year. Key words: beluga whale, white whale, Delphinapterus leucas , Gupuk, Kittigazuit, Mackenzie Inuit, Inuvialuit, Mackenzie Delta, zooarchaeology, archaeology
TL;DR: In this article, different combinations of biopsy tip lengths (20, 25 mm) and diameters (5, 6, 7 mm), crossbow draw weights (23, 45, 68 kg) and distances (1.5-15 m) were tested on fresh beluga carcasses to determine factors affecting the success of retrieval and the extent of wounding.
Abstract: Different combinations of biopsy tip lengths (20, 25 mm) and diameters (5, 6, 7 mm), crossbow draw weights (23, 45, 68 kg) and distances (1.5–15 m) were tested on fresh beluga carcasses to determine factors affecting the success of biopsy retrieval and the extent of wounding. Tips with smaller diameters and longer lengths were found to be more likely to retrieve a skin sample, while the draw weight of the crossbow had a significant effect on the severity of the wound. The samples obtained from all biopsy darts tested yielded sufficient amounts of DNA for genetic analysis (20–109 μg) with the highest yield coming from the germinativum spinosum layer. For beluga whales we recommend using a tip of 5 mm in diameter, 25 mm in length, and a draw weight of 23 kg at close range.