TL;DR: New single-chain ribosome-inactivating proteins (RIPs) were isolated from the seeds of Basella rubra L. and from the leaves of Bougainvillea spectabilis Willd, and both inhibited infection of Nicotiana benthamiana by AMCV.
Abstract: New single-chain (type 1) ribosome-inactivating proteins (RIPs) were isolated from the seeds of Basella rubra L. (two proteins) and from the leaves of Bougainvillea spectabilis Willd. (one protein). These RIPs inhibit protein synthesis both in a cell-free system, with an IC50 (concentration causing 50% inhibition) in the 10−10 M range, and by various cell lines, with IC50s in the 10−8–10−6 M range. All three RIPs released adenine not only from rat liver ribosomes but also from Escherichia coli rRNA, polyadenylic acid, herring sperm DNA, and artichoke mottled crinkle virus (AMCV) genomic RNA, thus being polynucleotide:adenosine glycosidases. The proteins from Basella rubra had toxicity to mice similar to that of most type 1 RIPs (Barbieri et al., 1993, Biochim Biophys Acta 1154: 237–282) with an LD50 (concentration that is 50% lethal) ≤ 8 mg · kg−1 body weight, whilst the RIP from Bougainvillea spectabilis had an LD50 >32 mg · kg−1. The N-terminal sequence of the two RIPs from Basella rubra had 80–93% identity, whereas it differed from the sequence of the RIP from Bougainvillea spectabilis. When tested with antibodies against various RIPs, the RIPs from Basella gave some cross-reactivity with sera against dianthin 32, and weak cross-reactivity with momordin I and momorcochin-S, whilst the RIP from Bougainvillea did not cross-react with any antiserum tested. An RIP from Basella rubra and one from Bougainvillea spectabilis were tested for antiviral activity, and both inhibited infection of Nicotiana benthamiana by AMCV.
TL;DR: In this paper, the authors used a co-chromatography and tandem mass spectrometry of the fresh juice from Basella rubra fruits to detect betanidin monoglucoside as the major betacyanin and its 4-coumaroyl derivatives as minor components.
TL;DR: The nutritional and antioxidant activity of two species of leafy vegetable Basella were investigated in this article, where the moisture content of Basella alba and Basella rubra leaves was 928 and 930 g/kg, respectively.
Abstract: The nutritional and antioxidant activity of two species of leafy vegetable Basella were investigated The moisture content of Basella alba and Basella rubra leaves was 928 and 930 g/kg, respectively The dietary fibre content constitutes 32 g, 10 g and 22 g total, soluble and insoluble dietary fibre, respectively, in 100 g of dry B alba leaves The leaves of Basella spp are very low in calories (95–110/100 g) and fats, but holds a good amount of vitamins, minerals, and antioxidants The betalains content in B rubra was highest in 20 weeks old leaves (80 mg) and vitamin C content of B alba was more (74 mg) than B rubra (67 mg) in 100 g fresh leaves The EC50 values were found high in BAW extract (34 mg/mL) for DPPH radical scavenging activity BAW extract (104) at 50 mg/mL showed high ferric reducing antioxidant power and least in BAM extract Various extracts from leaves with different constituents were shown dose dependent antioxidant and high scavenging abilities which play a key role in combating the reactive oxygen species In view of this, Basella spp could be an excellent example of 'functional food' that aims at lowering the risk of various diseases
TL;DR: Agarwal et al. as discussed by the authors have shown fruits from less-known and unexplored plants as an excellent source of nutrients and biologically active compounds for food and non-food applications.
Abstract: Recent studies have shown fruits from less-known and unexplored plants as an excellent source of nutrients and biologically active compounds for food and non-food applications (Khan et al. 2011, 2015). Basella plant is extremely heat tolerant and fast-growing perennial vine which is wildly cultivated as a cool-season vegetable. Basella alba is also known as Malabar spinach, Indian spinach, Ceylon spinach, climber spinach and vine spinach. In India, it is commonly known as “Poi”. Red-violet colour of leaves, stalks, petioles and fruits are due to the presence of betalains (Palada et al, 1999). This crop is suitable for both home and market garden in the lowland tropics. This plant is full of nutrition like normal spinach. Also, the oil obtained from its seeds can become the source of safe vegetable oil. This plant is good for health due to the presence of mineral, protein, oil, carbohydrate, fibre, carotenoid, organic acid, vitamins. In the winter season, Malabar spinach can be used in place of normal spinach as it has similar Nutritional and medicinal value. Basella plant species contain betacyanin, carotenoids, bioflavonoids, β-sitosterol and lupeol which are reported to have antioxidant, antiproliferative, antimicrobial, anti-inflammatory activities etc. and help in curing various diseases, namely anticancer, antiviral, antioxidant, anti-inflammatory, anti-cholesterol, anti-ulcer, antimicrobial, anti-hypoglycemic, wound healing, androgenic from the ancient times. Leaf juice is used in to treat catarrh and it is also an as safe laxative for children, pregnant women and in a urinary diseased patient. In Ayurveda Malabar spinach is called as “Upodika”, “Potaki”, “Malvaa”, “Amritvallari”, and in Siddha/Tamil as “Vaslakkirai” (Khare, 2004). It used as a vegetable in many countries like Philippines, Thailand China, Mongolia, India, Sri Lanka and many African countries. In India, In Andhra Pradesh curry of Basella and Yam is made popularly known as Kanda Bachali Koora, in Odisha, it is used to make Curries and Saaga.