TL;DR: This work reconstructs entire metabolic pathways for anthocyanins and carotenoids and their potential regulatory networks by detailed analysis of time-ordered gene co-expression networks and provides a cornerstone for understanding the underlying genetics governing flower timing and coloration and could accelerate selective breeding in azalea.
Abstract: Azaleas (Ericaceae) comprise one of the most diverse ornamental plants, renowned for their cultural and economic importance. We present a chromosome-scale genome assembly for Rhododendron simsii, the primary ancestor of azalea cultivars. Genome analyses unveil the remnants of an ancient whole-genome duplication preceding the radiation of most Ericaceae, likely contributing to the genomic architecture of flowering time. Small-scale gene duplications contribute to the expansion of gene families involved in azalea pigment biosynthesis. We reconstruct entire metabolic pathways for anthocyanins and carotenoids and their potential regulatory networks by detailed analysis of time-ordered gene co-expression networks. MYB, bHLH, and WD40 transcription factors may collectively regulate anthocyanin accumulation in R. simsii, particularly at the initial stages of flower coloration, and with WRKY transcription factors controlling progressive flower coloring at later stages. This work provides a cornerstone for understanding the underlying genetics governing flower timing and coloration and could accelerate selective breeding in azalea. Azaleas are one of the most diverse ornamental plants and have cultural and economic importance. Here, the authors report a chromosome-scale genome assembly for the primary ancestor of the azalea cultivar Rhododendro simsi and identify transcription factors that may function in flower coloration at different stages.
TL;DR: This is the first map of azalea up to the authors' knowledge and is thoroughly different than the recently published integrated maps and well-suited for mapping in a non-model crop.
Abstract: Background
Azalea (Rhododendron simsii hybrids) is the most important flowering pot plant produced in Belgium, being exported world-wide. In the breeding program, flower color is the main feature for selection, only in later stages cultivation related plant quality traits are evaluated. As a result, plants with attractive flowering are kept too long in the breeding cycle. The inheritance of flower color has been well studied; information on the heritability of cultivation related quality traits is lacking. For this purpose, QTL mapping in diverse genetic backgrounds appeared to be a must and therefore 4 mapping populations were made and analyzed.
TL;DR: Turfgrass established 2 years previously on an Enfield silt loam significantly suppressed the growth and development of all four species of shrubs as compared to plots where turfgrass was not a competitor.
Abstract: Planting of a shrub or tree into a mature grass sod may result in poor establishment of the woody species. This experiment was designed to measure the field response of four species of ornamental shrubs to turfgrass competition. The shrubs used were forsythia (Forsythia intermedia zabel), azalea (Rhododendron X), Japanese barberry (Berberis thunbergi DC.) and taxus (Taxus media Rend.). Turfgrass treatments included plots maintained at high or low rates of N fertilizer and receiving, in some cases, supplemental irrigation to maintain high soil moisture. Treatments with no turfgrass were either bark mulch or bare ground. Turfgrass plots were mowed regularly to a height of 7.6 cm. The effects of these variables were measured by evaluating several aspects of growth and development of each shrub species. Turfgrass established 2 years previously on an Enfield silt loam (Typic Distrochrepts) significantly suppressed the growth and development of all four species of shrubs as compared to plots where turfgrass was not a competitor. Differences in soil moisture or temperature are not believed to have been responsible for the differences observed in these findings. Plant competition for N was suggested by both the color ratings and analyses of leaf tissue of the shrubs during the first year of shrub establishment. Additional fertilizer, applied as a topdressing, was more beneficial to the turfgrass than to the shrubs, and did not significantly increase their growth in most cases. The addition of K and P did not increase growth in any of the treatments and apparently was not a factor in the competition between the grass and the shrubs.
TL;DR: It is demonstrated that the major factor for the determination of flower blooming is heat accumulation, i.e. a certain threshold of growing degree-days (GDD) index, and in particular, early spring flowers were sensitive to the accumulation of warm temperature than late-spring flowers.