Topic
Aurantimonas
About: Aurantimonas is a research topic. Over the lifetime, 16 publications have been published within this topic receiving 621 citations.
Papers
TL;DR: To accommodate this emerging coral pathogen, the creation of a new genus and species is proposed, Aurantimonas coralicida gen. nov., sp.
Abstract: A bacterium previously isolated from a diseased colony of the scleractinian coral Dichocoenia stokesi (common name elliptical star coral) was subjected to a detailed polyphasic taxonomic characterization. The isolate, designated WP1T, was halophilic and strictly aerobic and formed golden-orange-pigmented colonies after prolonged incubation. Cells of WP1T were Gram-negative, rod-shaped and showed a characteristic branching rod morphology. Chemotaxonomically, WP1T was characterized by having Q-10 as the major respiratory lipoquinone and sym-homospermidine as the main component of the cellular polyamine content. The predominant constituent in the cellular fatty acid profile was C18 : 1
ω7c, along with C19 : 0 cyclo ω8c and C16 : 0. Other fatty acids present in smaller amounts were C17 : 0, C18 : 0, C16 : 1
ω7c, C20 : 1
ω7c and C18 : 1 2-OH. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. Minor amounts of diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidyldimethylethanolamine were present. The G+C content of the genomic DNA was 66·3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that WP1T represents a separate subline of descent within the order ‘Rhizobiales’ of the ‘Alphaproteobacteria’. The new line of descent falls within the group of families that includes the Rhizobiaceae, Bartonellaceae, Brucellaceae and ‘Phyllobacteriaceae’, with no particular relative within this group. The 16S rRNA gene sequence similarity to all established taxa within this group was not higher than 92·0 % (to Mesorhizobium mediterraneum). To accommodate this emerging coral pathogen, the creation of a new genus and species is proposed, Aurantimonas coralicida gen. nov., sp. nov. (type strain WP1T=CIP 107386T =DSM 14790T).
254 citations
TL;DR: A new type of manganese-oxidizing enzyme has been identified in two alphaproteobacteria, “Aurantimonas manganoxydans” strain SI85-9A1 and Erythrobacter sp.
Abstract: A new type of manganese-oxidizing enzyme has been identified in two alphaproteobacteria, “Aurantimonas manganoxydans” strain SI85-9A1 and Erythrobacter sp. strain SD-21. These proteins were identified by tandem mass spectrometry of manganese-oxidizing bands visualized by native polyacrylamide gel electrophoresis in-gel activity assays and fast protein liquid chromatography-purified proteins. Proteins of both alphaproteobacteria contain animal heme peroxidase and hemolysin-type calcium binding domains, with the 350-kDa active Mn-oxidizing protein of A. manganoxydans containing stainable heme. The addition of both Ca2+ ions and H2O2 to the enriched protein from Aurantimonas increased manganese oxidation activity 5.9-fold, and the highest activity recorded was 700 μM min−1 mg−1. Mn(II) is oxidized to Mn(IV) via an Mn(III) intermediate, which is consistent with known manganese peroxidase activity in fungi. The Mn-oxidizing protein in Erythrobacter sp. strain SD-21 is 225 kDa and contains only one peroxidase domain with strong homology to the first 2,000 amino acids of the peroxidase protein from A. manganoxydans. The heme peroxidase has tentatively been named MopA (manganese-oxidizing peroxidase) and sheds new light on the molecular mechanism of Mn oxidation in prokaryotes.
141 citations
TL;DR: The results highlight the difficulty in utilizing 16S rRNA-based approaches to investigate the microbial ecology of Mn(II) oxidation and suggest that this clade of bacteria is widely distributed around the globe and may be important contributors to Mn cycling in many environments.
Abstract: Several closely related Mn(II)-oxidizing alpha-Proteobacteria were isolated from very different marine environments: strain SI85-9A1 from the oxic/anoxic interface of a stratified Canadian fjord, strain HTCC 2156 from the surface waters off the Oregon coast, and strain AE01 from the dorsal surface of a hydrothermal vent tubeworm. 16S rRNA analysis reveals that these isolates are part of a tight phylogenetic cluster with previously characterized members of the genus Aurantimonas. Other organisms within this clade have been isolated from disparate environments such as surface waters of the Arctic and Mediterranean seas, a deep-sea hydrothermal plume, and a Caribbean coral. Further analysis of all these strains revealed that many of them are capable of oxidizing dissolved Mn(II) and producing particulate Mn(III/IV) oxides. Strains SI85-9A1 and HTCC 2156 were characterized further. Despite sharing nearly identical 16S rRNA gene sequences with the previously described Aurantimonas coralicida, whole genome DNA-...
58 citations
TL;DR: Evidence from this polyphasic study showed that strain CW67(T) could not be assigned to any recognized species, and represents a novel species, for which the name Roseomonas frigidaquae sp.
Abstract: A motile, short rod-shaped and yellow-pigmented bacterium, designated strain CW5T, was isolated from a water-cooling system at Gwangyang, Republic of Korea. Cells were Gram-negative, facultatively anaerobic and catalase- and oxidase-positive. The major fatty acids were C18 : 1omega7c (64.7 %) and C16 : 0 (14.1 %). The DNA G+C content was 63.9 mol%. A phylogenetic tree based on 16S rRNA gene sequence comparison showed that strain CW5T clustered within the Aurantimonas lineage and is closely related to the type strains of Aurantimonas altamirensis (98.5 % sequence similarity) and Aurantimonas coralicida (95.7 %). The phenotypic characteristics and DNA-DNA hybridization data indicate that strain CW5T could be distinguished from the phylogenetic relatives A. altamirensis and A. coralicida. On the basis of the evidence presented in this study, strain CW5T represents a novel species of the genus Aurantimonas, for which the name Aurantimonas frigidaquae sp. nov. is proposed. The type strain is CW5T (=KCTC 12893T =JCM 14755T).
54 citations
TL;DR: In this paper, the polar lipid compositions of Aurantimonas and Fulvimarina were examined and it was shown that although the patterns were similar with regards the phospholipid and amino lipid compositions, there were clear differences in the presence or absence of the glycolipid sulfoquinovosyldiacylglycerol (SQDG).
Abstract: Members of the genera Aurantimonas and Fulvimarina have largely been described on the basis of 16S rRNA gene sequence analyses, biochemical tests and limited chemotaxonomic data. Examination of the polar lipid compositions of members of these two genera indicated that although the patterns were similar with regards the phospholipid and amino lipid compositions, there were clear differences in the presence or absence of the glycolipid sulfoquinovosyldiacylglycerol (SQDG). This glycolipid was absent in members of the species Aurantimonas coralicida and Fulvimarina pelagi but was present in Aurantimonas altamirensis, Aurantimonas ureilytica and Aurantimonas frigidaquae. These results are also consistent with the 16S rRNA sequence-based grouping. We therefore propose that the genus Aurantimonas be divided further to reflect this additional information. Thus, it is recommended that Aurantimonas altamirensis, Aurantimonas ureilytica and Aurantimonas frigidaquae are transferred to a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov. (type strain S21BT = CECT 7138T = CIP 109525T = KCTC 22106T = LMG 23375T = DSM 21988T), Aureimonas ureilytica comb. nov. (type strain 5715S-12T = KACC 11607T = DSM 18598T = CIP 109815T) and Aureimonas frigidaquae comb. nov. (type strain CW5T = KCTC 12893T = JCM 14755T = DSM 21987T). The type species of the new genus is Aureimonas altamirensis. Additionally, emended descriptions of the genera Aurantimonas and Fulvimarina are provided.
49 citations
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 2018 | 2 |
| 2017 | 3 |
| 2016 | 1 |
| 2011 | 3 |
| 2009 | 2 |