TL;DR: Evaluated nanostructured lipid system can be used in a strategy to improve the in vitro and in vivo anti-C.
Abstract: The genus Astronium (Anacardiaceae) includes species, such as Astronium fraxinifolium, Astronium graveolens, and Astronium urundeuva, which possess anti-inflammatory, anti-ulcerogenic, healing, and antimicrobial properties. Nanostructured lipid systems are able to potentiate the action of plant extracts, reducing the required dose and side effects and improving antimicrobial activity. This work aims to evaluate a nanostructured lipid system that was developed as a strategy to improve the anti-Candida albicans activity of hydroethanolic extracts of stems and leaves from Astronium sp. The antifungal activity against C. albicans (ATCC 18804) was evaluated in vitro by a microdilution technique. In addition to the in vitro assays, the Astronium sp. that showed the best antifungal activity and selectivity index was submitted to an in vivo assay using a model of vulvovaginal candidiasis infection. In these assays, the extracts were either used alone or were incorporated into the nanostructured lipid system (comprising 10% oil phase, 10% surfactant, and 80% aqueous phase). The results indicated a minimal inhibitory concentration of 125.00 µg/mL before incorporation into the nanostructured system; this activity was even more enhanced when this extract presented a minimal inhibitory concentration of 15.62 µg/mL after its incorporation. In vivo assay dates showed that the nanostructure-incorporated extract of A. urundeuva leaves was more effective than both the unincorporated extract and the antifungal positive control (amphotericin B). These results suggest that this nanostructured lipid system can be used in a strategy to improve the in vitro and in vivo anti-C. albicans activity of hydroethanolic extracts of Astronium sp.
TL;DR: A new genus is recognized on the basis of wind‐dispersed fruits from the Eocene of western North America and Miocene of eastern Asia, which have similarities to the extant Picrasma of the Simaroubaceae and are suggestive of possible affinities.
Abstract: A new genus is recognized on the basis of wind‐dispersed fruits from the Eocene of western North America and Miocene of eastern Asia. The fruits consist of an accrescent hypogynous calyx of five obovate sepals and one or more globose fruit bodies. Although the fossils were formerly placed in the extant genera Porana (Convolvulaceae) and Astronium (Anacardiaceae), our investigation of numerous specimens from several floras in the western United States (e.g., Florissant, Green River, Clarno) and Canada (Whipsaw Creek, British Columbia) and the Yilan and Shanwang floras of China reveals unique characters that indicate that the fossils are a distinct genus, which we name Chaneya. Unlike Porana and Astronium, the fossil calyces have stomata that are longitudinally aligned, and early stages of fruit development show a gynoecium of five apocarpous carpels, of which only one or two usually enlarge at maturity. Precise systematic placement of the fossil genus is uncertain, but similarities to the extant Picrasma o...
TL;DR: The evaluation of the number of positional isomers of gallotannins occurring in both Astronium species was obtained by high-performance liquid chromatography–electrospray ionization–ion trap mass spectrometry (HPLC/ESI-IT-MS).
Abstract: The species Astronium urundeuva (Allemao) Engl. and Astronium graveolens Jacq., which are used in Brazilian folk medicine to treat allergies, inflammation, diarrhea and ulcers, were investigated for their composition. The aim of this study was to define a rapid and reliable analytical approach, based on the flow-injection analysis-electrospray ionization-ion trap-tandem mass spectrometry (FIA-ESI-IT-MS-MS) and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF-MS), to investigate the full range of hydrolyzable tannins present in the extracts of these Astronium species. The MALDI-ToF-MS analysis allowed us to ascertain the presence of hydrolysable tannins in both Astronium species as a series of gallotannins with degrees of polymerization of 7 to 13 galloyl units. Moreover, the analysis by FIA-ESI-IT-MS-MS, as well as confirming this result and chemically defining gallotannins as galloylglucose compounds, highlighted the presence of further classes of hydrolysable tannins, such as hexahydrodiphenoyl esters of glucose and some gallic acid derivatives, providing information about their structure by a careful study of their fragmentation patterns. Finally, the evaluation of the number of positional isomers of gallotannins occurring in both Astronium species was obtained by high-performance liquid chromatography-electrospray ionization-ion trap mass spectrometry (HPLC/ESI-IT-MS). This is the first mass spectrometric evidence relating to the existence of gallotannins in Astronium genus.
TL;DR: Results showed that all tested wood species were classified as very resistant to both decay fungi, except the wood of Aspidosperma desmanthum, which demonstrated to be veryresistant to Pycnoporus sanguineous and resistant to Gloeophyllum trabeum.
Abstract: This study evaluated the natural resistance of six Amazonian wood species: Aspidosperma desmanthum (Araracanga) , Parinari excelsa (Parinari) , Mouriri callocarpa (Mirauba) , Marmaroxylon racemosum (Angelim-rajado) , Peltogyne paniculata (Roxinho) e Astronium sp. (Muiracatiara) against Pycnoporus sanguineous , a white rot fungus, and Gloeophyllum trabeum , a brown rot fungus. Testing was performed based on the American Society for Testing and Materials - Standard Method for Accelerated Laboratory Test of Natural Decay Resistance of Woods - ASTM D2017/81(86). Results showed that all tested wood species were classified as very resistant to both decay fungi, except the wood of Aspidosperma desmanthum , which demonstrated to be very resistant to Pycnoporus sanguineous and resistant to Gloeophyllum trabeum . The wood of Peltogyne paniculata showed the best performance against Pycnoporus sanguineous , whereas the wood of Astronium sp. presented the best results when submitted to Gloeophyllum trabeum attack.