Apple tree

Abstract: Low environmental temperatures promote anthocyanin accumulation and fruit colouration by up-regulating the expression of genes involved in anthocyanin biosynthesis and regulation in many fruit trees. However, the molecular mechanism by which fruit trees regulate this process in response to low temperature (LT) remains largely unknown. In this study, the cold-induced bHLH transcription factor gene MdbHLH3 was isolated from an apple tree and was found to interact physically and specifically through two regions (amino acids 1-23 and 186-228) at the N terminus with the MYB partner MdMYB1 (allelic to MdMYB10). Subsequently, MdbHLH3 bound to the promoters of the anthocyanin biosynthesis genes MdDFR and MdUFGT and the regulatory gene MdMYB1 to activate their expression. Furthermore, the MdbHLH3 protein was post-translationally modified, possibly involving phosphorylation following exposure to LTs, which enhanced its promoter-binding capacity and transcription activity. Our results demonstrate the molecular mechanism by which MdbHLH3 regulates LT-induced anthocyanin accumulation and fruit colouration in apple.

Abstract: The objective of this study was to study the structure and physicochemical properties of biochar derived from apple tree branches (ATBs), whose valorization is crucial for the sustainable development of the apple industry. ATBs were collected from apple orchards located on the Weibei upland of the Loess Plateau and pyrolyzed at 300, 400, 500 and 600 °C (BC300, BC400, BC500 and BC600), respectively. Different analytical techniques were used for the characterization of the different biochars. In particular, proximate and element analyses were performed. Furthermore, the morphological, and textural properties were investigated using scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, Boehm titration and nitrogen manometry. In addition, the thermal stability of biochars was also studied by thermogravimetric analysis. The results indicated that the increasing temperature increased the content of fixed carbon (C), the C content and inorganic minerals (K, P, Fe, Zn, Ca, Mg), while the yield, the content of volatile matter (VM), O and H, cation exchange capacity, and the ratios of O/C and H/C decreased. Comparison between the different samples show that highest pH and ash content were observed in BC500. The number of acidic functional groups decreased as a function of pyrolysis temperature, especially for the carboxylic functional groups. In contrast, a reverse trend was found for the basic functional groups. At a higher temperature, the brunauer–emmett–teller (BET) surface area and pore volume are higher mostly due to the increase of the micropore surface area and micropore volume. In addition, the thermal stability of biochars also increased with the increasing temperature. Hence, pyrolysis temperature has a strong effect on biochar properties, and therefore biochars can be produced by changing pyrolysis temperature in order to better meet their applications.

Abstract: The apple target recognition algorithm is one of the core technologies of the apple picking robot. However, most of the existing apple detection algorithms cannot distinguish between the apples that are occluded by tree branches and occluded by other apples. The apples, grasping end-effector and mechanical picking arm of the robot are very likely to be damaged if the algorithm is directly applied to the picking robot. Based on this practical problem, in order to automatically recognize the graspable and ungraspable apples in an apple tree image, a light-weight apple targets detection method was proposed for picking robot using improved YOLOv5s. Firstly, BottleneckCSP module was improved designed to BottleneckCSP-2 module which was used to replace the BottleneckCSP module in backbone architecture of original YOLOv5s network. Secondly, SE module, which belonged to the visual attention mechanism network, was inserted to the proposed improved backbone network. Thirdly, the bonding fusion mode of feature maps, which were inputs to the target detection layer of medium size in the original YOLOv5s network, were improved. Finally, the initial anchor box size of the original network was improved. The experimental results indicated that the graspable apples, which were unoccluded or only occluded by tree leaves, and the ungraspable apples, which were occluded by tree branches or occluded by other fruits, could be identified effectively using the proposed improved network model in this study. Specifically, the recognition recall, precision, mAP and F1 were 91.48%, 83.83%, 86.75% and 87.49%, respectively. The average recognition time was 0.015 s per image. Contrasted with original YOLOv5s, YOLOv3, YOLOv4 and EfficientDet-D0 model, the mAP of the proposed improved YOLOv5s model increased by 5.05%, 14.95%, 4.74% and 6.75% respectively, the size of the model compressed by 9.29%, 94.6%, 94.8% and 15.3% respectively. The average recognition speeds per image of the proposed improved YOLOv5s model were 2.53, 1.13 and 3.53 times of EfficientDet-D0, YOLOv4 and YOLOv3 and model, respectively. The proposed method can provide technical support for the real-time accurate detection of multiple fruit targets for the apple picking robot.