TL;DR: Results on lipid probe diffusion in dimyristoylphosphatidylcholine multibilayers indicated that at temperatures above the gel-to-liquid crystalline phase transition diffusion is isotropic, and analysis of video-FRAP data yielded diffusion coefficients consistent with those measured previously by using spot photobleaching.
Abstract: Spatially resolving light detectors allow, with proper calibration, quantitative analysis of the variations in two-dimensional intensity distributions over time. An ultrasensitive microfluorometer was assembled by using as a detector a microchannel plate-intensified video camera. The camera was interfaced with a software-based digital video analysis system to digitize, average, and process images and to directly control the timing of the experiments to minimize exposure of the specimen to light. The detector system has been characterized to allow its use as a photometer. A major application has been to perform fluorescence recovery after photobleaching measurements by using the camera in place of a photomultiplier tube (video-FRAP) with the goal of detecting possible anisotropic diffusion or convective flow. Analysis of the data on macromolecular diffusion in homogenous aqueous glycol solutions yielded diffusion constants in agreement with previous measurements. Results on lipid probe diffusion in dimyristoylphosphatidylcholine multibilayers indicated that at temperatures above the gel-to-liquid crystalline phase transition diffusion is isotropic, and analysis of video-FRAP data yielded diffusion coefficients consistent with those measured previously by using spot photobleaching. However, lipid probes in these multibilayers held just below the main phase transition temperature exhibited markedly anisotropic diffusive fluxes when the bleaching beam was positioned proximate to domain boundaries in the P beta' phase. Lipid probes and lectin receptor complexes diffused isotropically in fibroblast surface membranes with little evidence for diffusion channeled parallel to stress fibers. A second application was to trace the time evolution of cell surface reactions such as patching. The feasibility of following, on the optical scale, the growth of individual receptor clusters induced by the ligand wheat germ agglutinin was demonstrated.
TL;DR: In this paper, a gray scale image of a fingerprint composed of a field of pixels is converted to a binary image composed of pixels by a technique which takes into account the directivity of ridge and valley structure.
Abstract: A gray scale image of a fingerprint composed of a field of
pixels is converted to a binary image composed of a field of
pixels by a technique which takes into account the directivity of
the ridge and valley structure. Three intermediate binary im
ages are developed, one by the use of a vertical filter, one by
the use of a horizontal filter and a reference image by the use of
a filter which is not directionally biased. Corresponding sub
fields around each pixel in each of the three images are com
pared. If the subfield for the vertically derived image is closer to
that of the reference image then is the subfield for the horizon
tally derived image, then the binary value for the pixel from the
vertically derived image is used in the final image; and vice
versa. In this fashion, a fourth a final binary image is derived
from a combination of the vertically derived image and horizon
tally derived image which includes the best imagery from each
of those two intermediate images.
TL;DR: In this paper, the anisotropic rotational motion of the backbone and the side chains of poly(L-glutamic acid in the α-helical structure was investigated using the 13C-T1 and T2 relaxation times of all carbon atoms with directly attached protons.
Abstract: The anisotropic rotational motion of the backbone and the side chains of poly(L-glutamic acid) in the α-helical structure was investigated using the 13C-T1 and T2 relaxation times of all carbon atoms with directly attached protons, obtained at a 13C-Larmor frequency of 67.89 MHz. The evaluation of the nmr data was carried out according to the previously derived anisotropic diffusion model, in which the macromolecule is considered a rigid rod. The rotation of the backbone is characterized by two diffusion constants, D1 and D3, describing the rotation perpendicular to and around the symmetry axis. The additional internal motion of the Cβ-methylene group is described as a jump process with a jump rate, k1, between two allowed rotametric states. Steric considerations indicate that the occupation of the third rotameric position is forbidden. The rotation of the Cγ-methylene group is decribed as a one-dimensional diffusion process around the Cβ–Cγ bond. Investigation of the temperature dependence of the relaxation parameters led to the temperature dependence of the dynamic parameters. Activation energies were determined from these data. The dynamic parameters obtained for poly(L-glutamic acid) at 291 K are compared with the corresponding results of a previous study of poly(L-lysine).
The development of an anisotropic diffusion model for the motions of the rod-shaped poly(L-lysine) α-helix and its application to the interpretation of the 13C-relaxation data of this molecule have already been published previously. In this model, both the overall molecular tumbling and the various internal motions have been characterized by diffusion constants or jump rates typical for each process. These dynamic parameters can be calculated from the spin–lattice relaxation times, the spin–spin relaxation times and the NOE factors of the Cα, Cβ, and Cγ nuclei of the polypetide.
In the present paper, we describe the application of the above-mentioned dynamic model to the interpretation of 13C-relaxation studies of a further homopolypeptide, poly(L-glutamic acid), in the α-helical structure. Furthermore, we studied the temperature dependence of the relaxation times of this polymer and determined the anisotropic diffusion parameters at each temperature. From their temperature dependence and from comparison of our present results with the data of our previous study of poly(L-lysine), we were able to derive new insights into the intramolecular diffusion processes and the excitation of various motions.
TL;DR: An algorithm has been developed that allows a fast and efficient computation of reequilibration parameters and anisotropic diffusion can be taken into account.
Abstract: Reequilibration processes are often encountered within solids and they have long been described mathematically. However, computation of reequilibration data over entire processes is often difficult. An algorithm has been developed specifically for this purpose. It allows a fast and efficient computation of reequilibration parameters. Anisotropic diffusion can, moreover, be taken into account.
TL;DR: In this article, a diffusion model is presented which allows an experimental test for the existence of an anisotropic region at a crystal surface induced by the asymmetry of the vacuum-crystal interface.
Abstract: A diffusion model is presented which allows an experimental test for the existence of an anisotropic region at a crystal surface induced by the asymmetry of the vacuum-crystal interface. The model is a generalization of that developed by van Gorkum and Kornelsen1 which assumed a diffusion coefficient independent of position in the crystal. The model was modified by assuming that the anisotropy can be described by a variable diffusion coefficient very near the surface. It is shown that if such an anisotropic region exists, the fraction of trapped particles f should increase with the temperature at which the crystal is held during diffusion. If the region does not exist, the fraction of trapped particles should not depend on the crystal temperature. An attempt was made to measure the dependence of f on temperature for He trapping at very shallow He V traps in Mo (100). The results indicate that even at the depth of the shallowest traps in Mo (100) (≤ 5 A), no significant anisotropic diffusion of th...
TL;DR: In this article, the influence of weak disorder due to randomly distributed impurities is studied in a diagrammatic self-consistent approximation (SCDT) for quasi-one-dimensional (quasi-ld) and layered materials.
Abstract: For quasi-one-dimensional (quasi-ld) and layered materials, for highly anisotropic two-dimensional systems and wires the influence of weak disorder due to randomly distributed impurities is studied in a diagrammatic self-consistent approximation (SCDT). It is shown that for quasi-ld systems there is a threshold value, wc, for the interchain exchange integral. For w wc it is in a metallic state. In contrast with a quasi-ld case, for a layered material extended states in an upper part of energy band appear almost immediately when a weak interlayer tunneling is switched on. The mobility edge is determined as a function of the strength of this tunneling. An expression for the AC conductivity σ(ω) is found in both cases. For highly anisotropic 2d systems localization radii r‖ and r⊥ are determined and σ(ω) is found. A possible relevance of this model to a system of dislocations on the grain boundaries of a germanium bicrystal is noted. A model of a wire consisting of N parallel, weakly coupled chains is considered. It is shown that with an increase in interchain coupling a rather sharp transition from a localized state with a small localization radius (rc ∼ l, where l is the mean free-path) to a localized state with a large localization radius (rc ≃ Nl) should take place. A very important conclusion is that for any anisotropic system, the effect of anisotropy can be completely absorbed into anisotropic diffusion coefficients D‖, (ω) and D⊥ (ω) and that the ratio D⊥ (ω)/D ‖ (ω) remains constant for 0 < ωT < 1. The accuracy of SCDT is discussed. A short review of the present state of the theory of the metal-insulator transition in disordered systems is given.
TL;DR: In this paper, anisotropic diffusion in pure and diluted chains and in a fractal was used to obtain detailed dynamic scaling descriptions of anisotropy diffusion in chains and fractals.
Abstract: Exact scaling treatments are given of anisotropic diffusion in pure and diluted chains, and in a fractal. A dynamic decimation method is used to obtain detailed dynamic scaling descriptions. The results for chains show crossover to drift or localised behaviour, induced by anisotropy or dilution. The fractal results include bias-induced crossover, with exponent unity, from anomalous diffusion behaviour (exponent log2 5) to drift behaviour and scaling corrections (exponent log2 5/3) arising from rotational anisotropy.