Topic
Aeschynanthus radicans
About: Aeschynanthus radicans is a research topic. Over the lifetime, 5 publications have been published within this topic receiving 40 citations.
Papers
TL;DR: The results suggest that the regenerated plants have a stable ploidy level, and the regeneration method established in this study can be used for rapid propagation of ploidsy-stable Aeschynanthus radicans.
Abstract: Plant regeneration through direct somatic embryogenesis in Aeschynanthus radicans ‘Mona Lisa’ was achieved in this study. Globular somatic embryos were formed directly from cut edges of leaf explants and cut ends or on the surface of stem explants 4 wk after culture on Murashige and Skoog (MS) medium supplemented with N-phenyl-N′-1, 2, 3-thiadiazol-5-ylurea (TDZ) with α-naphthalene acetic acid (NAA), TDZ with 2,4-dichlorophenoxyacetic acid (2,4-D), or 6-benzylaminopurine (BA) or kintin (KN) with 2,4-D. MS medium containing 9.08 μM TDZ and 2.68 μM 2,4-D resulted in 71% of stem explants producing somatic embryos. In contrast, 40% of leaf explants produced somatic embryos when induced in medium containing 6.81 μM TDZ and 2.68 μM 2,4-D. Somatic embryos matured, and some germinated into small plants on the initial induction medium. Up to 64% of stem explants cultured on medium supplemented with 9.08 μM TDZ + 2.68 μM 2,4-D, 36% of leaf explants cultured on medium containing 6.81 μM TDZ and 2.68 μM 2,4-D had somatic embryo germination before or after transferring onto MS medium containing 8.88 μM BA and 1.07 μM NAA. Shoots elongated better and roots developed well on MS medium without growth regulators. Approximately 30–50 plantlets were regenerated from each stem or leaf explant. The regenerated plants grew vigorously after transplanting to a soil-less substrate in a shaded greenhouse with more than a 98% survival rate. Three months after their establishment in the shaded greenhouse, 500 plants regenerated from stem explants were morphologically evaluated, from which five types of variants that had large, orbicular, elliptic, small, and lanceolate leaves were identified. Flow cytometry analysis of the variants along with the parent showed that they all had one identical peak, indicating that the variant lines, like the parent, were diploid. The mean nuclear DNA contents of the variant lines and their parent ranged from 4.90 to 4.99 pg 2C−1, which were not significantly different statistically. The results suggest that the regenerated plants have a stable ploidy level, and the regeneration method established in this study can be used for rapid propagation of ploidy-stable Aeschynanthus radicans.
40 citations
2 Apr 2021
TL;DR: The purpose of this study was to observe the development phase of A.radicans flowers, determine the viability of pollen during flower development, and investigate the pollen storability at -20?C.
Abstract: . Damayanti F, Garvita RV, Wawangningrum H, Rahayu S. 2021. Flower development, pollen viability and pollen storage test of Aeschynanthus radicans. Biodiversitas 22: 1940-1945. Aeschynanthus radicans Jack. commonly-known as lipstick flowers, is an epiphytic plant in Gesneriaceae family. It has an attractive morphology and is easy to grow. Therefore A. radicans is used as mother species for hybridization by plant breeders. The flowers of A. radicans are dichogamous and strongly protandrous. The pollen matures before the stigma becomes receptive and bends away and down when the stigma becomes receptive. As a thought-after material for hybridization, little is known about the flower development, pollen viability, and storability. The purpose of this study was to observe the development phase of A.radicans flowers, determine the viability of pollen during flower development, and investigate the pollen storability at -20?C. Flower development was observed in phases from small buds to anthesis. Pollen viability was tested in 10%, 15%, and 20% sucrose solution for 4 hours. Pollen storage was tested at -20?C for 77 days and tested for viability every three weeks. The best sucrose concentration for the germination of fresh pollen was 20%. The highest pollen viability was at H0 was 58±3% and gradually decreased with flowerage. The lowest viability was 18±14% at flower stage H5, coinciding with the first day of stigma receptive. Pollen storage at -20?C decreased pollen viability to 30,2±2% after 42 days and to 13,54% after 77 days.
6 citations
Patent•
14 Jan 2009
TL;DR: In the improved aeschynanthuspulcher cultivation method, growing points are removed when the branches of the aeschid are ripe, 1g to 8g of brown sugar or cane sugar is added into each pot, and 100ppm to 300ppm of cycocel aqueous solution is sprayed on the surface of the leaves of the Aeschid as discussed by the authors.
Abstract: The invention belongs to the field of flower cultivation methods, in particular to the field of aeschynanthuspulcher cultivation methods. In the improved aeschynanthuspulcher cultivation method, growing points are removed when the branches of the aeschynanthuspulcher are ripe, 1g to 8g of brown sugar or cane sugar is added into each pot, and 100ppm to 300ppm of cycocel aqueous solution is sprayed on the surface of the leaves of the aeschynanthuspulcher. The method has the advantages that when the brown sugar and B9 are not added in, the flowers of aeschynanthuspulcher bloom 10cm to 15cm above the growing points, and are withered within 10 days to 15 days. When the brown sugar or the cane sugar and the cycocel are added in, all flowers above the growing points bloom, and the flowering period can be kept for more than 20 days.
3 citations
Patent•
3 Apr 2013
TL;DR: In this article, a cutting method for Aeschynanthus radicans is described, where the conditions are controlled to be suitable for aeschyna radicans cuttage under the premise that the number of radicans in the holes of the hole tray is reasonable.
Abstract: The invention discloses a cutting method for Aeschynanthus radicans. During Aeschynanthus radicans cuttage, the temperature is controlled to be 20 to 23 DEG C by day and 18 to 20 DEG C at night; the lighting is controlled to be 10,000 to 15,000 lux; the humidity is controlled to be 90 percent; and Aeschynanthus radicans are cut in a hole tray with 32 holes in such a manner that 7 to 8 Aeschynanthus radicans are cut in each hole. The method has the advantages that the conditions are controlled to be suitable for Aeschynanthus radicans cuttage; the number of Aeschynanthus radicans in the holes of the hole tray is reasonable; and under the premise that requirements suitable for the growth of Aeschynanthus radicans cuttage are met, rotten leaves are reduced obviously, and the cutting survival rate is improved.
Related Topics (5)
Performance Metrics
| Year | Papers |
|---|---|
| 2021 | 1 |
| 2020 | 1 |
| 2013 | 1 |
| 2009 | 2 |