Acetylcorynoline

Abstract: Objective: To isolate and purify corynoline and acetylcorynoline from Corydalis bungeana and develop a reversed phase HPLC method of determining the two components in C. bungeana . Method: Alkaloids were isolated from the ethanolic extract with column gel chromatography, and identified on the basis of spectral analysis (UV, 1H NMR, 13 C NMR) and physicochemical properties. For quantitative analysis of the two components, samples were separated on an ODS column with mobile phase of methanol 15 mmol·L -1 potassium dihydrogen phosphate/potassium phosphate dibasic (pH 6.70,70∶30). The flow rate was 0.8 mL·min -1 , and the detection was set at 289 nm. Result: The purity was 99.5% and 99.1% for corynoline and acetylcorynoline respectively. The calibration curves were linear in the range of 6.9～110.4 mg·L -1 corynoline and 8.7～139.5 mg·L -1 acetylcorynoline. The RSD was 2.1% and 2.7%,and the average recovery was 97.3% and 97.2% respectively. Conclusion: The method of isolating and purifying corynoline and acetylcorynoline from Corydalis bungeana and the HPLC method of simultaneous determination of the two components have been developed. The HPLC method is simple, easy to perform and applicable to the content determination of corynoline and acetylcorynoline in C. bungeana of various origins.[

Abstract: A simple and precise method using ion-pair high permance liquid chromatogra-phy was developed for the simultaneous determination of four isoquinoline alkaloids ,namelyprotopine,corynoline,acetylcorynoline and tetrahydrocoptisine in Corydalis bungeana Turez. A reversedphase system consisting of a chemically bonded ODS silica gel column and 0.025 mol·L-1 sodiumdihydrogen phosphate──methanol(35 :65)containing 0.1%sodium dodecyl sulphate(pH 8.0 as themobile phase was used.The four alkaloids were completely separated within 30 min. The analyticalresults for various samples were presented.