Journal Article10.1515/BCHM.1998.379.7.831
Yeast Elongation Factor 3: Structure and Function
26
TL;DR: The deduced amino acid sequence of EF-3 has revealed the presence of duplicated ATP-binding cassettes similar to those present in the membrane associated transporters and the existence of functional homologs ofEF-3 in higher eukaryotes is still an open question.
read more
Abstract: Elongation factor 3 (EF-3) is a unique and essential requirement of the fungal translational apparatus. EF-3 is a single polypeptide protein with a molecular weight of 116,000 required by yeast ribosomes for in vitro translation and for in vivo growth. The YEF3 gene, located on chromosome xii, is essential for the survival of yeast. The deduced amino acid sequence of EF-3 has revealed the presence of duplicated ATP-binding cassettes similar to those present in the membrane associated transporters. The carboxy-terminus of EF-3 contains blocks of lysine boxes essential for its functional interaction with yeast ribosomes. EF-3 stimulates binding of aminoacyl-tRNA to the ribosomal A-site by facilitating release of deacylated tRNA from the exit site (E-site). Chasing experiments revealed that EF-3 enhances the rate of tRNA dissociation from the E-site by a factor of two without affecting the affinity of the site for tRNA. EF-3 function is dependent on ATP hydrolysis. The existence of functional homologs of EF-3 in higher eukaryotes is still an open question. Further investigations are needed to settle this issue.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Inventory and function of yeast ABC proteins: about sex, stress, pleiotropic drug and heavy metal resistance.
TL;DR: This review provides a comprehensive discussion and update on the function and transcriptional regulation of all known ABC genes from yeasts, including those discovered in fungal pathogens.
289
Characterization of yeast extracellular vesicles: evidence for the participation of different pathways of cellular traffic in vesicle biogenesis.
Débora L. Oliveira,Ernesto S. Nakayasu,Ernesto S. Nakayasu,Luna S. Joffe,Allan J. Guimarães,Tiago J. P. Sobreira,Joshua D. Nosanchuk,Radames J. B. Cordero,Susana Frases,Arturo Casadevall,Igor C. Almeida,Leonardo Nimrichter,Marcio L. Rodrigues +12 more
TL;DR: The results suggest that both conventional and unconventional pathways of secretion are required for biogenesis of extracellular vesicles, which demonstrate the complexity of this process in the biology of yeast cells.
Tracing the structural evolution of eukaryotic ATP binding cassette transporter superfamily
TL;DR: A putative structural evolutionary path of eukaryote ABC transporters is presented that will increase the understanding on their origin, divergence and function.
The ATP-binding protein FgArb1 is essential for penetration, infectious and normal growth of Fusarium graminearum.
TL;DR: A novel insight into functions of the ABC transporter in fungal pathogenesis is provided and acetylation modification at sites K28, K65, K341 and K525 in FgArb1 is required for its biological functions.
56
Identification and Analysis of Chromodomain-Containing Proteins Encoded in the Mouse Transcriptome
Khairina Tajul-Arifin,Rohan D. Teasdale,Timothy Ravasi,David A. Hume,Gsl Members,John S. Mattick +5 more
TL;DR: The chromodomain is 40-50 amino acids in length and is conserved in a wide range of chromatic and regulatory proteins involved in chromatin remodeling, including 43 novel variants, some of which lack the chromodOMain.
References
Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
TL;DR: Related sequences in both alpha and beta and in other enzymes that bind ATP or ADP in catalysis help to identify regions contributing to an adenine nucleotide binding fold in both ATP synthase subunits.
5.6K
Codon usage patterns in Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster and Homo sapiens; a review of the considerable within-species diversity
Paul M. Sharp,Elizabeth Cowe,Desmond G. Higgins,Denis C. Shields,Kenneth H. Wolfe,Frank Wright +5 more
TL;DR: These trends for codon usage are illustrated for six species whereCodon usage has been examined in detail, by presenting the pooled codon used for the 10% of genes at either end of the major trend.
675
Chemical synthesis of biologically active oligoribonucleotides using beta-cyanoethyl protected ribonucleoside phosphoramidites.
TL;DR: It is demonstrated that the combination of standard DNA protecting groups, 5'-O-DMT, N-Bz (Ade and Cyt), N-iBu (Gua), beta-cyanoethyl for phosphate, in conjunction with TBDMS for 2'-hydroxyl protection, constitutes a reliable method for the preparation of fully active RNA.
352
Eukaryotic protein elongation factors.
TL;DR: In eukaryotes, peptide chain elongation is mediated by elongation factors EF-1 and EF-2, which are involved in functions other than protein synthesis, such as organization of the mitotic apparatus, signal transduction, developmental regulation, ageing and transformation.
339
Vanadium — an element in search of a role
TL;DR: Vanadium has recently begun to interest the biochemist, largely as a result of the discovery that it can act as a potent inhibitor of the sodium pump as mentioned in this paper. But vanadium's biological functions remain unknown.
319