Visualizing chromosome structure/organization
TL;DR: This review focuses on new approaches to studying chromatin organization and dynamics in plants, and on future prospects in unraveling the basic principle of chromosome organization.
read more
Abstract: With the rapid development of sequencing technologies in the past decade, many eukaryotic genomes have been resolved at the primary sequence level. However, organization of the genome within nuclei and the principles that govern such properties remain largely unclear. Optimization of fluorescence probe-based hybridization technologies combined with new advances in the instrumentation for microscopy has steadily yielded more structural information on chromosome organization in eukaryote model systems. These studies provide static snapshots of the detailed organization of chromatin. More recently, the successful application of a chromatin tagging strategy utilizing auto fluorescent fusion proteins opened a new era of chromatin studies in which the dynamic organization of the genome can be tracked in near real time. This review focuses on these new approaches to studying chromatin organization and dynamics in plants, and on future prospects in unraveling the basic principle of chromosome organization.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Chromosome territory arrangement and homologous pairing in nuclei of Arabidopsis thaliana are predominantly random except for NOR-bearing chromosomes
Ales Pecinka,Veit Schubert,Armin Meister,Gregor Kreth,Marco Klatte,Martin A. Lysak,Jörg Fuchs,Ingo Schubert +7 more
TL;DR: In this paper, the interphase chromosome arrangement in Arabidopsis thaliana has been analyzed and compared to Drosophila, showing that only the nucleolus organizing region (NOR)-bearing chromosome 2 and 4 homologs associate more often than randomly.
222
Centromere positioning and dynamics in living Arabidopsis plants.
Yuda Fang,David L. Spector +1 more
TL;DR: 4-D tracking of the entire set of centromeres through mitosis demonstrated that global centromere position is not precisely transmitted from the mother cell to daughter cells, providing important insight into the understanding of chromatin organization among different cells of a living organism.
Use of two-color fluorescence-tagged transgenes to study interphase chromosomes in living plants.
TL;DR: The overall findings are consistent with a random and largely static arrangement of interphase chromosomes in nuclei of root cells.
54
Gourds: Bitter, Bottle, Wax, Snake, Sponge and Ridge
N. P. S. Dhillon,Supannika Sanguansil,Sheo Pujan Singh,Mohammed Abu Taher Masud,Prashant Kumar,L. K. Bharathi,Halit Yetişir,Rukui Huang,Doan Xuan Canh,James D. McCreight +9 more
- 01 Jan 2016
TL;DR: The status of germplasm resources for sustained genetic improvement of these cucurbit species is reviewed and more accessions of these gourd species and their relatives need to be collected from various regions of the tropics, conserved, and evaluated to ensure continuous genetic gains in breeding programs.
39
Compartmentalization of the splicing machinery in plant cell nuclei.
Zdravko J. Lorković,Andrea Barta +1 more
TL;DR: Recent studies on nuclear organization of splicing factors in plant cells provide insights into the compartmentalization of the plant cell nuclei and conservation of nuclear compartments between plants and metazoans.
34
References
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
TL;DR: The use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations is described and chromosomes in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase spreads and interphase nuclei when human genomic DNA was used as a probe.
3.4K
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
Andrew J. Bannister,Philip Zegerman,Janet F. Partridge,Eric A. Miska,Jean O. Thomas,Robin C. Allshire,Tony Kouzarides +6 more
TL;DR: A stepwise model for the formation of a transcriptionally silent heterochromatin is provided: SUV39H1 places a ‘methyl marker’ on histone H3, which is then recognized by HP1 through its chromo domain, which may also explain the stable inheritance of theheterochromatic state.
3K
Chromosome territories, nuclear architecture and gene regulation in mammalian cells.
TL;DR: The emerging view is that chromosomes are compartmentalized into discrete territories and the location of a gene within a chromosome territory seems to influence its access to the machinery responsible for specific nuclear functions, such as transcription and splicing.
Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi.
Tom Volpe,Catherine A. Kidner,Ira M. Hall,Ira M. Hall,Grace Teng,Grace Teng,Shiv I. S. Grewal,Robert A. Martienssen +7 more
TL;DR: It is proposed that double-stranded RNA arising from centromeric repeats targets formation and maintenance of heterochromatin through RNAi.
2.2K
Nuclear architecture and gene regulation in mammalian cells
Christoph Cremer
- 01 Jan 2001
TL;DR: The emerging view is that chromosomes are compartmentalized into discrete territories, and the location of a gene within a chromosome territory seems to influence its access to the machinery responsible for specific nuclear functions, such as transcription and splicing.
2.1K