Journal Article10.1002/HED.24968
Use of oral mucosal cell sheets for accelerated oral surgical wound healing.
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TL;DR: A highly efficient in vitro‐engineered mucosa equivalent using completely autologous mucosa and blood is developed and investigated its feasibility and efficacy for oral surgical wound healing.
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Abstract: Background
We developed a highly efficient in vitro-engineered mucosa equivalent using completely autologous mucosa and blood and investigated its feasibility and efficacy for oral surgical wound healing.
Methods
Small oral mucosa samples were obtained from surgical patients, and keratinocytes and fibroblasts were primarily grown in media without animal products for generating 3D cell sheets.
Results
Morphological characteristics of the cell sheets were comparable to those of human mucosa, although p63-positive cells were more numerous in cell sheets. In addition, cell sheets were flexible, expandable, and easy to handle or transfer. In further in vivo rat experiments with deep wounding of the buccal mucosa and soft tissues, controls had significantly thinner epithelium and thicker collagen densities than those with cell sheets.
Conclusion
Autologous cell sheets can be engineered in vitro from oral keratinocytes, fibroblasts, and fibrin, and can be used clinically to accelerate healing of oral soft tissue defects.
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Citations
Oral wound healing models and emerging regenerative therapies.
TL;DR: A review of the literature on in vivo oral wound healing models and emerging regenerative therapies published during the past twenty years is presented in this article, which discusses current gold standards for oral mucosal wound healing and compares endogenous factors that dictate the quality of tissue remodeling.
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Development of an in vitro cell-sheet cancer model for chemotherapeutic screening.
TL;DR: The 3D cell sheet-based cancer model developed using in vitro cell-sheet engineering could be applied to in vitro observation of epithelial cancer growth and invasion and to anticancer drug testing.
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Cancer-Associated Fibroblasts: The Origin, Biological Characteristics and Role in Cancer—A Glance on Colorectal Cancer
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TL;DR: Tumor microenvironment is a major contributor to tumor growth, metastasis and resistance to therapy, which consists of many cancer-associated fibroblasts (CAFs), which derive from different types of cells.
Tissue Engineering: What is New?
TL;DR: Soft and hard tissue engineering has expanded the frontiers of oral/maxillofacial augmentation by improving flap prevascularization and using stem cells and other cells to create not only the graft, but also the vascularization and soft tissue scaffolding for the graft.
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Identification of the Potential Biomarkers Involved in the Human Oral Mucosal Wound Healing: A Bioinformatic Study
Wanchen Ning,Xiao Jiang,Zhengyang Sun,Anthony Chukwunonso Ogbuehi,Wenli Gu,Aneesha Acharya,Zhaobi Fang,Xiongjie Zhu,Qianhua Ou,Muhui Zeng,Cong Li,Shiting Hua,Prabhakar Mujagond,Xiangqiong Liu,Yupei Deng,Hongying Pan,Shaonan Hu,Xianda Hu,Simin Li +18 more
TL;DR: Genetic and epigenetic mechanisms and specific drugs were identified as significant molecular mechanisms and entities relevant to oral mucosa regeneration by regulating the expression of healing-associated DEGs/miRNAs.
References
Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.
James G. Rheinwatd,Howard Green +1 more
TL;DR: Human diploid epidermis epidermal cells have been successfully grown in serial culture and it is possible to isolate keratinocyte clones free of viable fibroblasts, and human diploids keratinocytes appear to have a finite culture lifetime.
4.4K
p63 identifies keratinocyte stem cells
Graziella Pellegrini,Elena Dellambra,Osvaldo Golisano,Enrica Martinelli,Ivana Fantozzi,Sergio Bondanza,Diego Ponzin,Frank McKeon,Michele De Luca +8 more
TL;DR: It is shown by clonal analysis that p63 is abundantly expressed by epidermal and limbal holoclones, but is undetectable in paraclones, and will be of practical importance for the clinical application of epithelial cultures in cell therapy as well as for studies on epithelial tumorigenesis.
Three clonal types of keratinocyte with different capacities for multiplication
Yann Barrandon,Howard Green +1 more
TL;DR: Colony-forming human epidermal cells are heterogeneous in their capacity for sustained growth and the incidence of the different clonal types is affected by aging, since cells originating from the epidermis of older donors give rise to a lower proportion of holoclones and a higher proportion of paraclones.
1.3K
Human plasma as a dermal scaffold for the generation of a completely autologous bioengineered skin.
Sara Llames,Marcela Del Rio,Fernando Larcher,Eva García,Marta García,María José Escámez,José L. Jorcano,Purificación Holguín,Alvaro Meana +8 more
TL;DR: A new cultured bioengineered skin based on keratinocytes and fibroblasts obtained from a single skin biopsy and a dermal matrix based on human plasma is described, demonstrating that this new dermal equivalent allows for generation of large bioengineering skin surfaces, restoration of both the epidermal and dermal skin compartments, and functional epidersmal stem-cell preservation.
218
Development and Characterization of a Tissue-engineered Human Oral Mucosa Equivalent Produced in a Serum-free Culture System
TL;DR: Ex vivo assembly of a human oral mucosa equivalent, consisting of epidermal and dermal components, in a defined, essential-fatty-acid-deficient, serum-free culture medium without a feeder layer, that could be used for intra-oral grafting in humans is successful.
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