Three-dimensional optical method for integrated visualization of mouse islet microstructure and vascular network with subcellular-level resolution
Ya-Yuan Fu,Chih-Hsuan Lu,Chi-Wen Lin,Jyuhn-Huarng Juang,Grigori Enikolopov,Eric Sibley,Ann-Shyn Chiang,Shiue-Cheng Tang +7 more
TL;DR: An optical method for penetrative imaging of mouse islets yielded clear, continuous optical sections for an integrated visualization of the islet microstructure and vasculature with subcellular-level resolution, providing a useful imaging approach to change the conventional planar view of the Islet structure into a 3-D panorama for better understanding of theIslet physiology.
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Abstract: Microscopic visualization of islets of Langerhans under nor- mal and diabetic conditions is essential for understanding the patho- physiology of the disease. The intrinsic opacity of pancreata, however, limits optical accessibility for high-resolution light microscopy of islets in situ. Because the standard microtome-based, 2-D tissue analysis confines visualization of the islet architecture at a specific cut plane, 3-D representation of image data is preferable for islet assessment. We applied optical clearing to minimize the random light scattering in the mouse pancreatic tissue. The optical-cleared pancreas allowed pen- etrative, 3-D microscopic imaging of the islet microstructure and vas- culature. Specifically, the islet vasculature was revealed by vessel painting—lipophilic dye labeling of blood vessels—for confocal mi- croscopy. The voxel-based confocal micrographs were digitally pro- cessed with projection algorithms for 3-D visualization. Unlike the microtome-based tissue imaging, this optical method for penetrative imaging of mouse islets yielded clear, continuous optical sections for an integrated visualization of the islet microstructure and vasculature with subcellular-level resolution. We thus provide a useful imaging approach to change our conventional planar view of the islet structure into a 3-D panorama for better understanding of the islet physiology. © 2010 Society of Photo-Optical Instrumentation Engineers. DOI: 10.1117/1.3470241
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Citations
Recent progress in tissue optical clearing
TL;DR: Using the tissue optical clearing technique with advanced microscopy image or labeling technique, applications for 3D microstructure of whole tissues such as brain and central nervous system with unprecedented resolution are demonstrated.
Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy
Elina A. Genina,Alexey N. Bashkatov,Yury P. Sinichkin,Irina Yu. Yanina,Valery V. Tuchin +4 more
- 28 Mar 2015
TL;DR: The possibility of efficient control of the biotissue optical properties, particularly, the reduction of light scattering in biotissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes.
Advances in Ex Situ Tissue Optical Clearing
TL;DR: The progress in TOC for peripheral organs is delineated in an organ‐by‐organ fashion and whole‐body clearing approaches are discussed and physical and optical approaches for TOC affecting the optical properties of the samples and image quality are discussed to explore their advantages, limitations, and future possibilities.
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3-D imaging and illustration of mouse intestinal neurovascular complex.
TL;DR: 3-D imaging approach provides a useful tool to simultaneously reveal the nerves and blood vessels in a space continuum for panoramic illustration and analysis of the neurovascular complex to better understand the intestinal physiology and diseases.
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3-D imaging, illustration, and quantitation of enteric glial network in transparent human colon mucosa
Yuan An Liu,Y. C. Chung,S. T. Pan,M. Y. Shen,Y. C. Hou,S. J. Peng,Pankaj J. Pasricha,S. C. Tang +7 more
TL;DR: This work prepared transparent human colon mucosa for three‐dimensional (3‐D) confocal microscopy with S100B immunostaining to reveal the location‐dependent glial network for qualitative and quantitative analyses.
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