The Role of the CopA Copper Efflux System in Acinetobacter baumannii Virulence.
Saleh F. Alquethamy,Marjan Khorvash,Victoria G. Pederick,Jonathan J. Whittall,Jonathan J. Whittall,James C. Paton,Ian T. Paulsen,Karl A. Hassan,Christopher A. McDevitt,Bart A. Eijkelkamp +9 more
TL;DR: This study performs an in silico analysis of the A. baumannii copper resistome, examining its regulation under copper stress, and reveals that CopA contributes to the virulence of A. Baumanni pneumonia.
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Abstract: Acinetobacter baumannii has emerged as one of the leading causative agents of nosocomial infections. Due to its high level of intrinsic and adapted antibiotic resistance, treatment failure rates are high, which allows this opportunistic pathogen to thrive during infection in immune-compromised patients. A. baumannii can cause infections within a broad range of host niches, with pneumonia and bacteraemia being associated with the greatest levels of morbidity and mortality. Although its resistance to antibiotics is widely studied, our understanding of the mechanisms required for dealing with environmental stresses related to virulence and hospital persistence, such as copper toxicity, is limited. Here, we performed an in silico analysis of the A. baumannii copper resistome, examining its regulation under copper stress. Using comparative analyses of bacterial P-type ATPases, we propose that A. baumannii encodes a member of a novel subgroup of P1B-1 ATPases. Analyses of three putative inner membrane copper efflux systems identified the P1B-1 ATPase CopA as the primary mediator of cytoplasmic copper resistance in A. baumannii. Using a murine model of A. baumannii pneumonia, we reveal that CopA contributes to the virulence of A. baumannii. Collectively, this study advances our understanding of how A. baumannii deals with environmental copper toxicity, and it provides novel insights into how A. baumannii combats adversities encountered as part of the host immune defence.
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Bacterial adaptive strategies to cope with metal toxicity in the contaminated environment - A review.
Krishnamurthy Mathivanan,Jayaraman Uthaya Chandirika,Annadurai Vinothkanna,Huaqun Yin,Xueduan Liu,Delong Meng +5 more
TL;DR: In this paper, the authors present a comprehensive review of the prevailing strategies of adaptive/resistance mechanisms in bacterial bioremediation, and their respective future directions are enumerated in invigorating effective biOREmediation technologies including overexpression studies and delivery systems.
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Critical Review: Propensity of Premise Plumbing Pipe Materials to Enhance or Diminish Growth of Legionella and Other Opportunistic Pathogens.
Abraham C. Cullom,Rebekah L. Martin,Rebekah L. Martin,Yang Song,Krista Williams,Amanda J. Williams,Amy Pruden,Marc Edwards +7 more
TL;DR: All known pipe materials have a potential to either stimulate or inhibit OP growth, depending on the circumstances, and this review delineates some of these circumstances and informs future research and guidance towards effective deployment of pipe materials for control of OPs.
Role of Glutathione in Buffering Excess Intracellular Copper in Streptococcus pyogenes.
Louisa J Stewart,Cheryl-lynn Y. Ong,May M. Zhang,Stephan Brouwer,Liam McIntyre,Mark R. Davies,Mark J. Walker,Alastair G. McEwan,Kevin J. Waldron,Karrera Y. Djoko +9 more
TL;DR: This work with group A Streptococcus provides new evidence that glutathione increases the threshold of intracellular Cu availability that can be tolerated by bacteria and thus advances fundamental understanding of bacterial Cu handling.
Characterization of Acinetobacter baumannii Copper Resistance Reveals a Role in Virulence.
Caitlin L. Williams,Heather M. Neu,Yonas Alamneh,Ryan M. Reddinger,Anna C. Jacobs,Shweta Singh,Rania Abu-Taleb,Sarah L. J. Michel,Daniel V. Zurawski,D. Scott Merrell +9 more
TL;DR: Copper possesses antimicrobial activity against multidrug-resistant A. baumannii, and copper sensitivity is further increased when copper homeostasis mechanisms are interrupted, including copper transport, oxidation, sequestration, and regulation.
49
Stabilization of supramolecular membrane protein-lipid bilayer assemblies through immobilization in a crystalline exoskeleton.
Fabian C. Herbert,Sameera S. Abeyrathna,Nisansala S. Abeyrathna,Yalini H. Wijesundara,Olivia R. Brohlin,Francesco Carraro,Heinz Amenitsch,Paolo Falcaro,Michael A. Luzuriaga,Alejandra Durand-Silva,Shashini D. Diwakara,Ronald A. Smaldone,Gabriele Meloni,Jeremiah J. Gassensmith +13 more
TL;DR: In this article, the authors demonstrate that metastable lipid, protein-detergent, and protein-lipid supramolecular complexes can be successfully generated and immobilized within the zeolitic-imidazole framework (ZIF) to enhance their stability against chemical and physical stressors.
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