The Polycomb-Dependent Epigenome Controls β Cell Dysfunction, Dedifferentiation, and Diabetes.
Tess Tsai Hsiu Lu,Steffen Heyne,Erez Dror,Eduard Casas,Laura Leonhardt,Laura Leonhardt,Thorina Boenke,Chih-Hsiang Yang,Sagar,Laura Arrigoni,Kevin Dalgaard,Raffaele Teperino,Lennart Enders,Madhan Selvaraj,Marius Ruf,Sunil Jayaramaiah Raja,Huafeng Xie,Ulrike Boenisch,Stuart H. Orkin,Francis C. Lynn,Brad G. Hoffman,Dominic Grün,Tanya Vavouri,Adelheid Lempradl,J. Andrew Pospisilik +24 more
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TL;DR: Deep epigenome mapping with single-cell transcriptomics finds two chromatin-state signatures that track β cell dysfunction in mice and humans: ectopic activation of bivalent Polycomb-silenced domains and loss of expression at an epigenomically unique class of lineage-defining genes.
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About: This article is published in Cell Metabolism. The article was published on 05 Jun 2018. and is currently open access. The article focuses on the topics: Epigenome & Chromatin.
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Citations
Advances in epigenetics link genetics to the environment and disease
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TL;DR: The authors review recent advances and current debates in epigenetics, including how epigenetic mechanisms interact with genetic variation, ageing, disease and the environment.
Charting cellular identity during human in vitro β-cell differentiation
Adrian Veres,Aubrey L. Faust,Henry L. Bushnell,Elise N. Engquist,Jennifer H. R. Kenty,George Harb,Yeh Chuin Poh,Elad Sintov,Mads Gürtler,Felicia W. Pagliuca,Quinn P. Peterson,Douglas A. Melton,Douglas A. Melton +12 more
TL;DR: A perspective on human stem-cell differentiation is provided, and will guide future endeavours that focus on the differentiation of pancreatic islet cells, and their applications in regenerative medicine.
484
Histone H3.3 K27M Accelerates Spontaneous Brainstem Glioma and Drives Restricted Changes in Bivalent Gene Expression.
Jon D. Larson,Lawryn H. Kasper,Barbara S. Paugh,Hongjian Jin,Gang Wu,Chang-Hyuk Kwon,Yiping Fan,Timothy I. Shaw,Andre B. Silveira,Chunxu Qu,Raymond Xu,Xiaoyan Zhu,Junyuan Zhang,Helen R. Russell,Jennifer L. Peters,David Finkelstein,Beisi Xu,Tong Lin,Christopher L. Tinkle,Zoltan Patay,Arzu Onar-Thomas,Stanley Pounds,Peter J. McKinnon,David W. Ellison,Jinghui Zhang,Suzanne J. Baker +25 more
TL;DR: Genetically engineered inducible mice were generated and it was shown that H3.3 K27M enhanced neural stem cell self-renewal while preserving regional identity and upregulated genes were enriched for those encoding homeodomain transcription factors.
281
PRC2 is high maintenance
Jia Ray Yu,Jia Ray Yu,Chul Hwan Lee,Chul Hwan Lee,Ozgur Oksuz,Ozgur Oksuz,James M. Stafford,James M. Stafford,Danny Reinberg,Danny Reinberg +9 more
TL;DR: In this review, Yu et al. discuss the recent advances in knowledge of the Polycomb-repressive complex 2 (PRC2) in mammalian systems and discuss important discoveries on Polycomb function derived from model organisms in the context of understanding mammalian PRC2 function.
Leader β-cells coordinate Ca2+ dynamics across pancreatic islets in vivo.
Victoria Salem,Luis F. Delgadillo Silva,Kinga Suba,Eleni Georgiadou,S Neda Mousavy Gharavy,Nadeem Akhtar,Aldara Martin-Alonso,David C. A. Gaboriau,Stephen Rothery,Theodoros Stylianides,Gaelle Carrat,Timothy J. Pullen,Sumeet Pal Singh,David J. Hodson,David J. Hodson,Isabelle Leclerc,A. M. James Shapiro,Piero Marchetti,Linford J.B. Briant,Walter Distaso,Nikolay Ninov,Guy A. Rutter +21 more
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TL;DR: In this paper, the authors used the recombinant Ca2+ sensor GCaMP6 to assess glucose-regulated connectivity in living zebrafish Danio rerio, and in murine or human islets transplanted into the anterior eye chamber.
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