Journal Article10.1034/J.1398-9995.2003.00036.X
The effect of storage on allergen and microbial agent levels in frozen house dust.
B. Fahlbusch,A. Koch,Jeroen Douwes,Wolfgang Bischof,Ulrike Gehring,Klaus Richter,Heinz Erich Wichmann,Joachim Heinrich +7 more
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TL;DR: In this paper, the effects of dust storage on the content of biocontaminants were investigated in the context of a study on the influences of INdoor factors and Genetics on Asthma (INGA).
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Abstract: Background: House dust samples collected for exposure studies are often stored for variable time periods until analysis. However, there is currently no information on the effects of dust storage on the content of biocontaminants. Therefore, associations were analysed between the levels of mite allergens (Der p 1, Der f 1), cat allergen (Fel d 1) and microbial components (endotoxin, β(13)-glucan) on the one hand and the storage duration of dust samples at −20°C on the other hand.
Methods: Within the framework of a study on the influences of INdoor factors and Genetics on Asthma (INGA), dust samples were collected from living room floors between June 1995 and August 1998 and extracted according to a standardized protocol. The concentrations of Der p 1, Der f 1, Fel d 1 and β(13)-glucan were determined with specific enzyme immunoassays. Endotoxin content was quantified using a chromogenic kinetic Limulus amoebocyte lysate (LAL) test. All concentrations were expressed per gram of dust
Results: Dust samples (n = 1236) were obtained from 655 homes in Hamburg, Hettstedt, Zerbst and Bitterfeld. Storage duration (range 8–298 days) was grouped into four categories (≤ 60 d; 61–90 d; 91–120 d; > 120 d). After adjustment for city of residence and season of dust sampling, means ratios comparing categories 2–4 to the first category were not statistically significant for Der p 1, Der f 1, endotoxin and β(13 glucan). However, Fel d 1 concentrations significantly declined with increased storage times of dust samples.
Conclusions: Storage of house dust at −20°C for up to 10 months has no effect on mite allergen, endotoxin and β(13)-glucan levels. A potential loss of Fel d 1 during storage of frozen dust samples needs further investigations by repeated measurements of allergen in identical dust samples.
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Citations
Orchestrating house dust mite-associated allergy in the lung
Lisa G. Gregory,Clare M. Lloyd +1 more
TL;DR: This work reviews the myriad ways in which HDM allergic responses are orchestrated and describes the molecular pathways that elicit HDM-associated pathology, likely to reveal novel targets for therapeutic intervention.
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Exposure to inhalable dust and endotoxins in agricultural industries
TL;DR: Overall, exposure to endotoxins over the whole range of agricultural industries is high and a 10-1,000 fold reduction is needed to reduce endotoxin related health risks.
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Endoplasmic reticulum stress mediates house dust mite-induced airway epithelial apoptosis and fibrosis
Sidra M. Hoffman,Jane E. Tully,James D. Nolin,Karolyn G. Lahue,Dylan Goldman,Nirav Daphtary,Minara Aliyeva,Charles G. Irvin,Anne E. Dixon,Matthew E. Poynter,Vikas Anathy +10 more
TL;DR: It is indicated that HDM induces ER stress in airway epithelial cells and that ATF6α and ERp57 play a significant role in the development of cardinal features of allergic airways disease.
Protease-activated receptor-2 deficient mice have reduced house dust mite-evoked allergic lung inflammation:
J. Daan de Boer,Cornelis van 't Veer,Ingrid Stroo,Anne Jan van der Meer,Alex F. de Vos,Jaring S. van der Zee,Joris J. T. H. Roelofs,Tom van der Poll +7 more
TL;DR: This study reveals, for the first time, an important role for PAR2 in allergic lung inflammation induced by the clinically relevant allergens contained in HDM.
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Geographical variation and the determinants of domestic endotoxin levels in mattress dust in Europe.
Cm Chen,E. Thiering,Gert Doekes,J P Zock,Ioannis Bakolis,Dan Norbäck,Jordi Sunyer,Simona Villani,Giuseppe Verlato,Martin Täubel,Deborah Jarvis,Deborah Jarvis,Joachim Heinrich +12 more
TL;DR: In this article, the authors investigated the geographical variation and determinants of domestic endotoxin concentrations in mattress dust in Europe using data collected in the European Community Respiratory Health Survey follow-up (ECRHS II).
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