Book Chapter10.1016/B978-0-12-814020-8.00005-6
The Disk Angiogenesis System
Domenico Ribatti
- 01 Jan 2018
pp 55-59
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TL;DR: The implant system consists of 11 mm disks of polyvinyl alcohol foam, covered on both sides by Millipore filters, leaving only the edge as the area for cell penetration in the disk, which shows a rich neovascularization and a distinctive cellular infiltration at the edges.
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Abstract: The implant system consists of 11 mm disks of polyvinyl alcohol foam, covered on both sides by Millipore filters, leaving only the edge as the area for cell penetration in the disk. Angiogenic factors or antagonists, as well as other substances to be studied, are placed in the center of the disk. The disk is implanted subcutaneously in the host animal through a distal skin incision. Histological examination of sections of the disk shows a rich neovascularization and a distinctive cellular infiltration at the edges, including fibroblasts, endothelial cells, and leukocytes. Throughout the area of growth the majority of cells are fibroblasts, the second most common cells are endothelial cells. Electron microscopy can also be used alone or in combination with light microscopy. The visualization of vessels can be improved with intravascular dyes, inert substances, or microspheres.
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Citations
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The Rat Subcutaneous Air Sac Model: A New and Simple Method for <i>in vivo</i> Screening of Antiangiogenesis
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References
•Journal Article
Transforming growth factor beta1 induces angiogenesis in vivo with a threshold pattern.
TL;DR: TGFbeta1 is angiogenic in vivo, when it reaches a threshold of 1 microg, but is notAngiogenic at doses of 1 to 500 ng, while the combined effect of TGFbeta and fibroblast growth factor was studied by using suboptimal doses of both.
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•Journal Article
The disc angiogenesis system.
TL;DR: The disc angiogenesis system is easy to prepare, inexpensive, and well tolerated, at least by mice, and its simplicity and reproducibility make it suitable for a wide range of applications beyond those described here.
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•Journal Article
Factors Influencing Blood Supply in Wound Granuloma Quantitated by a New in Vivo Technique
TL;DR: Using this model, it has been shown that the known angiogenic factors, transforming growth factor alpha and tumor necrosis factor alpha, cause maximum vascularization of the sponge to occur by Day 11 postimplantation compared with Days 15 to 17 in control animals.
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Characterization and applications of the disc angiogenesis system
TL;DR: Using the DAS, well-established angiogenic agents such as basic fibroblast growth factor (bFGF), epidermal growth factor, and prostaglandin E1 were found to increase proliferation of endothelial cells (EC) and microvessels, and Heparin augmented the effect of bFGF.
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Effects of X irradiation on angiogenesis.
Stavros D. Prionas,Joe Kowalski,Luis F. Fajardo,Irving Kaplan,Helen H. Kwan,Anthony C. Allison +5 more
TL;DR: Investigation of mesenchymal tissue growth and previous observations point to endothelial cells as important targets of ionizing radiation in the stroma, especially during the period of active proliferation of these cells, induced by growth factors.
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