Journal Article10.1038/74542
Targeted screening for induced mutations
861
TL;DR: The TILLING (Targeting Induced Local Lesions IN Genomes) method combines the efficiency of ethyl methanesulfonate (EMS)-induced mutagenesis with the ability of denaturing high-performance liquid chromatography (DHPLC) to detect base pair changes by heteroduplex analysis.
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Abstract: With the accumulation of large-scale sequence data, emphasis in genomics has shifted from determining gene structure to testing gene function, and this relies on reverse genetic methodology. Here we explore the feasibility of screening for chemically induced mutations in target sequences in Arabidopsis thaliana. Our TILLING (Targeting Induced Local Lesions IN Genomes) method combines the efficiency of ethyl methanesulfonate (EMS)-induced mutagenesis1 with the ability of denaturing high-performance liquid chromatography (DHPLC) to detect base pair changes by heteroduplex analysis2. Importantly, this method generates a wide range of mutant alleles, is fast and automatable, and is applicable to any organism that can be chemically mutagenized.
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References
Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques
Salah M. Aljanabi,Iciar Martinez +1 more
TL;DR: The quantity and the quality of the DNA extracted by this method is high enough to perform hundreds of PCR-based reactions and also to be used in other DNA manipulation techniques such as restriction digestion, Southern blot and cloning.
2.9K
Genes directing flower development in Arabidopsis.
TL;DR: It seems that the wild-type alleles of these four genes allow cells to determine their place in the developing flower and thus to differentiate appropriately and is proposed that these genes may be involved in setting up or responding to concentric, overlapping fields within the flower primordium.
1.2K
Detection of Numerous Y Chromosome Biallelic Polymorphisms by Denaturing High-Performance Liquid Chromatography
Peter A. Underhill,Li Jin,Alice A. Lin,S. Qasim Mehdi,Trefor Jenkins,Douglas Vollrath,Ronald W. Davis,Luigi Luca Cavalli-Sforza,Peter J. Oefner +8 more
TL;DR: It is now possible to anticipate the inevitable detailed reconstruction of human Y chromosome genealogy based on several tens to even hundreds of these important polymorphisms.
Mutation detection using a novel plant endonuclease
TL;DR: The results indicate that CEL I mutation detection is a highly sensitive method for detecting both polymorphisms and disease-causing mutations in DNA fragments as long as 1120 bp in length.
427
EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh.
TL;DR: Average mutation frequencies per locus per dose of mutagen — EMS, X-rays and fast neutrons — were estimated from 15 different loci of Arabidopsis thaliana to reject the proposition of a linear relationship between DNA content and mutation frequency per loci.
375
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