Suppression of antibody and T cell proliferative responses to L-glutamic acid60-L-alanine30-L-tyrosine10 by a specific monoclonal T cell factor.
TL;DR: Experiments reported in this manuscript show that a hybrid T cell line, produced by fusion of the AKR thymoma with spleen cells that contain Gat-specific suppressor T cells, produces a constitutive GAT-specific suppresor factor that functionally and serologically resembles GAT -TsF extracted from T cells.
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Abstract: The synthetic terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) stimulates GAT-specific suppressor T cells in nonresponder mice. Extracts from these T cells contain a GAT-specific soluble T cell suppressor factor (GAT-TsF) that inhibits development of GAT-specific plaque-forming cell (PFC) responses by spleen cells from nonresponder mice stimulated with GAT complexed to methylated bovine serum albumin (GAT-MBSA). These extracts also contain a factor that inhibits development of GAT-specific proliferative responses by GAT-MBSA-primed, nonresponder lymph node T cells. Experiments reported in this manuscript show that a hybrid T cell line, produced by fusion of the AKR thymoma, BW5147, with spleen cells that contain GAT-specific suppressor T cells, produces a constitutive GAT-specific suppresor factor that functionally and serologically resembles GAT-TsF extracted from T cells. More importantly, both GAT-specific PFC and T cell proliferative responses are inhibited by this factor.
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TL;DR: Four of five suppressor T-cell hybridomas examined have deleted all known joining (Jβ) gene segments and Cβ genes and therefore may have antigen receptors encoded by different T- cell receptor gene families.
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References
Antibodies to major histocompatibility antigens produced by hybrid cell lines.
TL;DR: FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies.
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•Journal Article
Immunosuppressive Factor(s) Extracted from Lymphoid Cells of Nonresponder Mice Primed with L-Glutamic Acid60-L-Alanine30-L-Tyrosine10 (GAT) I. Activity and Antigenic Specificity
TL;DR: A suppressive factor can be extracted from lymphoid cells of G AT-primed nonresponder mice that inhibits the development of primary GAT-specific antibody responses to GAT -MBSA and to Gat-PRBC- by normal syngeneic mice.
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T-cell lines producing antigen-specific suppressor factor
Sirkka Kontiainen,Elizabeth M. Simpson,Elaine Bohrer,P. C. L. Beverley,Leonore A. Herzenberg,W. C. Fitzpatrick,Peter Vogt,A. Torano,Ian F. C. McKenzie,Marc Feldmann +9 more
TL;DR: One antigen-specific suppressor factor (SF)-producing line S1-41 and its products are described, particularly useful as it is monoclonal and large quantities can be obtained.
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Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) III. Immunochemical properties of the GAT-specific suppressive factor.
TL;DR: The G AT-specific suppressor T-cell factor extracted from lymphoid cells from GAT-primed, nonresponder DBA/1 mice has been partially characterized and resembles anti-GAT-MBSA antibodies produced by DBA/, in spite of the fact that it is too small to be classical antibody and has no constant-region determinants of heavy or light chains.
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Specific suppressive factors produced by hybridomas derived from the fusion of enriched suppressor T cells and a T lymphoma cell line
Masaru Taniguchi,Jfap Miller +1 more
TL;DR: Preliminary results with the T lymphoma-sensitive hypoxanthine aminopterin thymidine cell line, L5178, indicate retention of the expression of surface markers derived from the normal parent for 18 wk after hybidization, suggesting that T lymphomas cell lines may have to be screened for their capacity to produce hybridomas with stable properties.
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