Journal Article10.1088/1758-5090/AA74F2
Study of gelatin as an effective energy absorbing layer for laser bioprinting.
TL;DR: The objectives of this study are to investigate the feasibility of using gelatin as an energy absorbing layer (EAL) material for laser bioprinting and its effects on the quality of printed constructs, bioink printability, and post-printing cell viability and process-induced DNA damage.
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Abstract: Laser-induced forward transfer printing, also commonly known as laser printing, has been widely implemented for three-dimensional bioprinting due to its unique orifice-free nature during printing. However, the printing quality has the potential to be further improved for various laser bioprinting applications. The objectives of this study are to investigate the feasibility of using gelatin as an energy absorbing layer (EAL) material for laser bioprinting and its effects on the quality of printed constructs, bioink printability, and post-printing cell viability and process-induced DNA damage. The gelatin EAL is applied between the quartz support and the coating of build material, which is to be printed. Printing quality can be improved by EAL-assisted laser printing when using various alginate solutions (1%, 2%, and 4%) and cell-laden bioinks (2% alginate and 5 × 106 cells ml−1 in cell culture medium). The required laser fluence is also reduced due to a higher absorption coefficient of gelatin gel, in particular when to achieve the best printing type/quality. The post-printing cell viability is improved by ~10% and DNA double-strand breaks are reduced by ~50%. For all the build materials investigated, the gelatin EAL helps reduce the droplet size and average jet velocity.
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Citations
3D bioprinting of functional tissue models for personalized drug screening and in vitro disease modeling
TL;DR: This review presents state-of-the-art 3D bioprinting techniques and discusses the choice of cell source and biomaterials for building functional tissue models that can be used for personalized drug screening and disease modeling.
341
Bio-inks for 3D bioprinting: recent advances and future prospects
Ilze Donderwinkel,Ilze Donderwinkel,Jcm Jan van Hest,Jcm Jan van Hest,Neil R. Cameron,Neil R. Cameron +5 more
TL;DR: All the currently used bio-printing inks, including polymeric hydrogels, polymer bead microcarriers, cell aggregates and extracellular matrix proteins, and photocrosslinkable and thermoresponsive materials are described.
313
Polymeric Systems for Bioprinting.
TL;DR: A review of recent bioprinting innovations, such as increasing architectural complexity and cell viability in heterogeneous tissue constructs, which allow for the investigation of biological questions that could not be addressed before.
214
Bioinks and bioprinting: A focused review
TL;DR: This review will feature recent advances in this field, focusing on bioink developments and bioprinting applications, and the use of natural and synthetic additives that impart unique, novel, or critical functionalities.
194
Current Status in the Utilization of Biobased Polymers for 3D Printing Process: A Systematic Review of the Materials, Processes, and Challenges
Mahdiyar Shahbazi,Henry Jäger +1 more
- 18 Jan 2021
TL;DR: Three-dimensional (3D) printing is a revolutionary additive manufacturing technique that allows rapid prototyping of objects with intricate architectures that help in the design and construction of complex objects.
130
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