SSHscreen and SSHdb, generic software for microarray based gene discovery: application to the stress response in cowpea.
TL;DR: The SSHscreen-SSHdb software pipeline is developed, which greatly facilitates gene discovery using suppression subtractive hybridization by improving the selection of clones for sequencing after screening the library on a small number of microarrays.
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Abstract: Background
Suppression subtractive hybridization is a popular technique for gene discovery from non-model organisms without an annotated genome sequence, such as cowpea (Vigna unguiculata (L.) Walp). We aimed to use this method to enrich for genes expressed during drought stress in a drought tolerant cowpea line. However, current methods were inefficient in screening libraries and management of the sequence data, and thus there was a need to develop software tools to facilitate the process.
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Recent advances in cowpea [ Vigna unguiculata (L.) Walp.] “omics” research for genetic improvement
TL;DR: In this article, a significant amount of omics datasets are available and useful in understanding the genetic relationship between cowpea ssp. unguiculata and other species belonging to the same genus as well as its genetic variation.
Universal Stress Protein Exhibits a Redox-Dependent Chaperone Function in Arabidopsis and Enhances Plant Tolerance to Heat Shock and Oxidative Stress.
Young Jun Jung,Sarah Mae Boyles Melencion,Eun Seon Lee,Joung Hun Park,Cresilda Vergara Alinapon,Hun Taek Oh,Dae-Jin Yun,Yong Hun Chi,Sang Yeol Lee +8 more
TL;DR: Plants over-expressing AtUSP showed a strong resistance to heat shock and oxidative stress, compared with wild-type and Atusp knock-out plants, confirming the crucial role of At USP in stress tolerance.
Identification of Drought-Responsive Universal Stress Proteins in Viridiplantae
Raphael D. Isokpehi,Shaneka S. Simmons,Hari H. P. Cohly,Stephen I N Ekunwe,Gregorio Begonia,Wellington K. Ayensu +5 more
TL;DR: The datasets from cross-database mining provide organized resources for the characterization of USP genes as useful targets for engineering plant varieties tolerant to unfavorable environmental conditions.
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Twenty-Five Years of Investigating the Universal Stress Protein: Function, Structure, and Applications.
Amy Cheng Vollmer,Steven J. Bark +1 more
TL;DR: The objective has been to organize the foundational studies in this field to identify the significant impediments to further understanding of Usp functions at the molecular level and propose ideas and experimental approaches that may overcome these impediments and drive future development of molecular approaches to understand and target Usps as central regulators of stress adaptation and survival.
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High-density genetic linkage maps with over 2,400 sequence-anchored DArT markers for genetic dissection in an F2 pseudo-backcross of Eucalyptus grandis × E. urophylla
Anand Raj Kumar Kullan,Maria M. Van Dyk,Nicoletta B. Jones,Arnulf Kanzler,Arlene Bayley,Alexander Andrew Myburg +5 more
TL;DR: An integrated genetic linkage map will allow detailed genetic dissection of quantitative traits and hybrid fitness characters segregating in the F2 progeny and will facilitate the development of markers for molecular breeding in Eucalyptus.
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