Book Chapter10.1007/978-3-540-68182-3_4
Special Engineering Aspects
Peter Czermak,Ralf Pörtner,Alexander Brix +2 more
- 01 Jan 2009
- pp 83-172
TL;DR: This chapter starts with a detailed discussion of shear stress effects on mammalian cells in model systems and bioreactors, respectively, and consequences for reactor design.
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Abstract: The specific characteristics of mammalian cells discussed in Chap. 2 require adapted solutions for bioreactor design and operation. Especially, cell damage by shear stress and aeration has to be considered. Therefore this chapter starts with a detailed discussion of shear stress effects on mammalian cells (anchorage-dependent and suspendable cells) in model systems and bioreactors, respectively, and consequences for reactor design. Appropriate oxygen supply is another critical issue, as adapted oxygen supply systems are required. Techniques for immobilization of cells, either grown on microcarriers in suspension culture or within macroporous carriers in fixed bed or fluidized bed reactors, are discussed as well. With respect to the operation of bioreactors, the characteristics of different culture modes (batch, fed-batch, chemostat, perfusion) are introduced and practical examples are given. Finally, concepts for monitoring of bioreactors, including offline and online methods as well as control loops (e.g. O2, pH), are considered.
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Citations
Mesenchymal Stem Cells as a Cornerstone in a Galaxy of Intercellular Signals: Basis for a New Era of Medicine.
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TL;DR: In this paper, the mesenchymal stem cells (MSC) have been used as a therapeutic tool in the field of regenerative medicine, since they reproduce the effects of their parent cells.
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Dynamic Single-Use Bioreactors Used in Modern Liter- and m 3 - Scale Biotechnological Processes: Engineering Characteristics and Scaling Up
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68
Alginate as an immobilization material for MAb production via encapsulated hybridoma cells.
S. Mert Selimoglu,Murat Elibol +1 more
TL;DR: This review discuses and clarifies some complicated aspects of cell immobilization using alginate biopolymers, using as an example the bioprocessing of highly fragile cells (hybridoma cells).
45
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