Small-angle scattering and its interplay with crystallography, contrast variation in SAXS and SANS.
TL;DR: Methods of contrast variation are tools that are essential in macromolecular structure research and recent attempts to extend this method to native resonant labels like sulfur and phosphorus are promising.
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Abstract: Methods of contrast variation are tools that are essential in macromolecular structure research. Anomalous dispersion of X-ray diffraction is widely used in protein crystallography. Recent attempts to extend this method to native resonant labels like sulfur and phosphorus are promising. Substitution of hydrogen isotopes is central to biological applications of neutron scattering. Proton spin polarization considerably enhances an existing contrast prepared by isotopic substitution. Concepts and methods of nuclear magnetic resonance (NMR) become an important ingredient in neutron scattering from dynamically polarized targets.
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Citations
Characterization and Analysis of Porosity and Pore Structures
TL;DR: There are a large number of methods for quantifying porosity, and an increasingly complex idea of what it means to do so as discussed by the authors, which is why it is important to quantify the relationships between porosity and storage, transport and rock properties, however, the pore structure must be measured and quantitatively described.
Nanoporous carbon for electrochemical capacitive energy storage
Hui Shao,Hui Shao,Yih-Chyng Wu,Yih-Chyng Wu,Zifeng Lin,Pierre-Louis Taberna,Pierre-Louis Taberna,Patrice Simon,Patrice Simon,Patrice Simon +9 more
TL;DR: This review aims at summarizing the recent progress in nanoporous carbons, as the most commonly used EDLC electrode materials in the field of capacitive energy storage, from the viewpoint of materials science and characterization techniques.
487
Combining structure and dynamics: non-denaturing high-pressure effect on lysozyme in solution
Maria Grazia Ortore,Francesco Spinozzi,Paolo Mariani,Alessandro Paciaroni,Leandro R.S. Barbosa,Heinz Amenitsch,Milos Steinhart,Jacques Ollivier,Daniela Russo +8 more
TL;DR: Small-angle X-ray scattering (SAXS) and elastic and quasi-elastic neutron scattering techniques were used to investigate the high-pressure-induced changes on interactions, the low-resolution structure and the dynamics of lysozyme in solution, and protein structure and dynamics results have been discussed in the context of protein–water interface and hydration water dynamics.
Hybrid Biopolymer and Lipid Nanoparticles with Improved Transfection Efficacy for mRNA.
Christian D Siewert,Heinrich Haas,Vera Cornet,Sara S. Nogueira,Thomas Nawroth,Lukas Uebbing,Antje Ziller,Jozef Al-Gousous,Aurel Radulescu,Martin A. Schroer,Clement E. Blanchet,Dmitri I. Svergun,Markus P. Radsak,Ugur Sahin,Peter Langguth +14 more
TL;DR: The systems with the highest transfection efficacy were characterized by a heterogenous internal organization, accompanied by facilitated release, and could be best obtained by the single step protocol, starting with a lipid and polymer mixture for nanoparticle formation.
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References
Restoring low resolution structure of biological macromolecules from solution scattering using simulated annealing.
TL;DR: Application of the method is illustrated by the restoration of a ribosome-like model structure and more realistically by the determination of the shape of several proteins from experimental x-ray scattering data.
2.3K
Three-dimensional model of purple membrane obtained by electron microscopy
Richard Henderson,P. N. T. Unwin +1 more
TL;DR: A 7-Å resolution map of the purple membrane has been obtained by electron microscopy of tilted, unstained specimens and shows that Lipid bilayer regions fill the spaces between the protein molecules.
2.3K
Structure of the 30S ribosomal subunit.
Brian T. Wimberly,Ditlev E. Brodersen,William M. Clemons,William M. Clemons,Robert J. Morgan-Warren,Andrew P. Carter,Clemens Vonrhein,Thomas Hartsch,Venki Ramakrishnan +8 more
TL;DR: The crystal structure of the 30S subunit from Thermus thermophilus, refined to 3 Å resolution, is reported, which will facilitate the interpretation in molecular terms of lower resolution structural data on several functional states of the ribosome from electron microscopy and crystallography.
2.1K
Uniqueness of ab initio shape determination in small-angle scattering
TL;DR: In this article, scattering patterns from geometrical bodies with different shapes and anisometry (solid and hollow spheres, cylinders, prisms) are computed and the shapes are reconstructed using envelope function and bead modelling methods.