Journal Article10.1007/S00294-020-01134-3
Single-molecule fluorescence microscopy reveals modulation of DNA polymerase IV-binding lifetimes by UmuD (K97A) and UmuD′
Sarah S. Henrikus,Sarah S. Henrikus,Sarah S. Henrikus,Antoine M. van Oijen,Antoine M. van Oijen,Andrew Robinson,Andrew Robinson +6 more
TL;DR: In this article, the UmuD (K97A) protein was investigated as a potential modulator of DNA polymerase IV (pol IV) activity in ciprofloxacin-treated cells.
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Abstract: DNA polymerase IV (pol IV) is expressed at increased levels in Escherichia coli cells that suffer DNA damage. In a recent live-cell single-molecule fluorescence microscopy study, we demonstrated that the formation of pol IV foci is strongly recB-dependent in cells treated with the DNA break-inducing antibiotic ciprofloxacin. The results of that study support a model in which pol IV acts to extend D-loop structures during recombinational repair of DNA double-strand breaks. In the present study, we extend upon this work, investigating the UmuD and UmuD' proteins as potential modulators of pol IV activity in ciprofloxacin-treated cells. We found that the non-cleavable mutant UmuD(K97A) promotes long-lived association of pol IV with the nucleoid, whereas its cleaved form, UmuD', which accumulates in DNA-damaged cells, reduces binding. The results provide additional support for a model in which UmuD and UmuD' directly modulate pol IV-binding to the nucleoid.
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Regulation of SOS mutagenesis by proteolysis
TL;DR: These phenotypes are consistent with the suggestion that while the UmuD/D' heterodimer is mutagenically inactive, it still retains the ability to interact with UmuC, and thereby precludes the formation of themutagenically active UmUD'2C complex.
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John P. Pribis,Libertad García-Villada,Yin Zhai,Ohad Lewin-Epstein,Anthony Z. Wang,Jingjing Liu,Jun Xia,Qian Mei,Devon M. Fitzgerald,Julia Bos,Robert H. Austin,Christophe Herman,David Bates,Lilach Hadany,P. J. Hastings,Susan M. Rosenberg +15 more
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