Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer
Fei Wang,J Long,Liang Li,Zixin Wu,Tian-Tian Da,Xiao-Qing Wang,Chuan Huang,Yi-Hua Jiang,Xueqing Yao,Haijun Ma,Zhe-Xiong Lian,Zhi-Bin Zhao,Jie Cao +12 more
TL;DR: In this paper , the authors comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing.
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Abstract: In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45− nonimmune cells and 196,473 CD45+ immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. F3+ fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, MCAM+ fibroblasts enriched in liver metastatic tumors might promote generation of CD8_CXCL13 cells through Notch signaling. In summary, we extensively analyzed the transcriptional differences of cell atlas between primary and liver metastatic tumors of CRC by single-cell and spatial transcriptome RNA sequencing, providing different dimensions of the development of liver metastasis in CRC.
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Mechanisms of metastatic colorectal cancer
Adrià Cañellas‐Socias,Elena Sancho,Eduard Batlle +2 more
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Advances in spatial transcriptomics and its applications in cancer research
Jin Yang,Yuanli Zuo,Gang Luo,Wenrong Liu,Yitong Pan,Fan Tang,Xin Fu,Xiaojun Yao,Yong Peng +8 more
TL;DR: Spatial transcriptomics technologies enable the quantification and visualization of gene expression in the spatial context of tissues, providing valuable insights into tumor biology and development.
Characterization of immune cell populations in the tumor microenvironment of colorectal cancer using high definition spatial profiling
Michelli F. Oliveira,Juan Pablo Romero,Matthew Chung,Steven C.R. Williams,Andrew D. Gottscho,Anushka Gupta,Susan E. Pilipauskas,Syrus Mohabbat,Nandhini Raman,David J. Sukovich,David Patterson,Samuel J. Taylor +11 more
- 05 Jun 2024
TL;DR: High-definition spatial profiling of immune cell populations in the tumor microenvironment of colorectal cancer reveals distinct subpopulations and their localization within the tumor periphery.
High-definition spatial transcriptomic profiling of immune cell populations in colorectal cancer.
Meii Chung,Andrew D. Gottscho,Susan E. Pilipauskas,Seayar Mohabbat,Nandhini Raman,David Sukovich,David Patterson +6 more
TL;DR: Researchers employed high-definition spatial transcriptomics to profile immune cell populations in colorectal cancer, identifying distinct macrophage subpopulations with pro-tumor and anti-tumor functions, and characterizing their interactions with tumor cells and T cells.
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Progressive plasticity during colorectal cancer metastasis
Andrew R Moorman,Elizabeth K. Benitez,Francesco Cambuli,Qiang Jiang,Amir Mahmoud,Melissa Lumish,Saskia Hartner,Sandy Balkaran,Jonathan Bermeo,Simran Asawa,Cemal Fırat,Asha Krishnan,Fan Wu,A. Luthra,Cassandra Burdziak,Yubin Xie,Valeria Sgambati,Kathleen Luckett,Yun Li,Zhengjun Yi,Ignas Masilionis,Kevin C. Soares,Emmanouil P. Pappou,Rona Yaeger,T. Peter Kingham,William R. Jarnagin,Philip B. Paty,Martin R. Weiser,Linas Mažutis,M.I. D’Angelica,Jinru Shia,Julio García‐Aguilar,Tal Nawy,Ronan Chaligné,Ronan Chaligné,Francisco Sánchez-Vega,Roshan Sharma,Dana Pe’er,Karuna Ganesh +38 more
TL;DR: Colorectal cancer metastasis exhibits progressive plasticity, with cancer cells transitioning from intestinal stem-like states to a fetal progenitor state, then to squamous and neuroendocrine-like states, driven by loss of PROX1-mediated repression and exacerbated by chemotherapy.
19
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