Sequential Multiplex Analyte Capturing for Phosphoprotein Profiling
Oliver Poetz,Tanja Henzler,Michael Hartmann,Michael Hartmann,Cornelia Kazmaier,Markus F. Templin,Thomas Herget,Thomas O. Joos +7 more
38
TL;DR: The value of sequential multiplex analyte capturing was demonstrated by probing tumor cell line lysates for the abundance of seven different receptor tyrosine kinases and their degree of phosphorylation and by measuring the complexosphorylation pattern of the epidermal growth factor receptor in the same sample from the same cavity.
read more
About: This article is published in Molecular & Cellular Proteomics. The article was published on 03 Aug 2010. and is currently open access. The article focuses on the topics: Analyte & Multiplex.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
The Human Protein Atlas as a proteomic resource for biomarker discovery
TL;DR: The Human Protein Atlas as a proteomic resource for biomarker discovery and its applications in medicine and drug discovery are reviewed.
289
Cross-reactivity in antibody microarrays and multiplexed sandwich assays: shedding light on the dark side of multiplexing
TL;DR: This work reviews CR and its consequences in single and dual antibody single-plex and multiplex assays, establishing a distinction between sample-driven and reagent-driven CR, and describes how it affects the performance of antibody microarrays.
143
Sequential Multiplexed Analyte Quantification Using Peptide Immunoaffinity Enrichment Coupled to Mass Spectrometry
TL;DR: The ability to sequentially enrich sets of analyte peptides was demonstrated by enriching groups of 10 peptides from a plasma sample in a sequential fashion, demonstrating the utility of immuno-SRM for analyzing large numbers of analytes, such as in large biomarker verification experiments or in pathway-based targeted analysis.
75
Systematic antibody and antigen-based proteomic profiling with microarrays
Burcu Ayoglu,Anna Häggmark,Maja Neiman,Ulrika Igel,Mathias Uhlén,Jochen M. Schwenk,Peter Nilsson +6 more
TL;DR: Some of the current building blocks within array- and affinity-based proteomic profiling with a focus on body fluids are summarized, by giving a perspective on how current and upcoming developments in this bioscience could enable a path of pursuit for biomarker discovery.
68
Liquid crystal-based immunoassays for detecting hepatitis B antibody.
Chih-Hsin Chen,Kun-Lin Yang +1 more
TL;DR: A liquid crystal (LC)-based immunoassay that features a new signal transduction mechanism, whereby the test results appear as colorful spots and can be viewed with the naked eye directly, making it more suitable for point-of-care diagnosis of hepatitis B in resource-limited regions.
43
References
Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Jesper V. Olsen,Blagoy Blagoev,Florian Gnad,Boris Macek,Boris Macek,Chanchal Kumar,Peter Mortensen,Matthias Mann +7 more
TL;DR: A general mass spectrometric technology is developed and applied for identification and quantitation of phosphorylation sites as a function of stimulus, time, and subcellular location to provide a missing link in a global, integrative view of cellular regulation.
3.7K
•Book
A comprehensive pathway map of epidermal growth factor receptor signaling
Kanae Oda,Yukiko Matsuoka,Akira Funahashi,Hiroaki Kitano +3 more
- 01 Jan 2005
TL;DR: A comprehensive pathway map of EGFR signaling and other related pathways is presented and reveals that the overall architecture of the pathway is a bow‐tie (or hourglass) structure with several feedback loops.
1.2K
Ubiquitin ligase activity and tyrosine phosphorylation underlie suppression of growth factor signaling by c-Cbl/Sli-1.
Gil Levkowitz,Hadassa Waterman,Seth A. Ettenberg,Menachem Katz,Alexander Y. Tsygankov,Iris Alroy,Sara Lavi,Kazuhiro Iwai,Yuval Reiss,Aaron Ciechanover,Stanley Lipkowitz,Yosef Yarden +11 more
TL;DR: This study identifies Cbl proteins as components of the ubiquitin ligation machinery and implies that they similarly suppress many other signaling pathways.
1K
Phosphotyrosine interactome of the ErbB‐receptor kinase family
TL;DR: The results demonstrate that system‐wide mapping of peptide‐protein interactions sites is possible, and suggest shared and unique roles of ErbB‐receptor family members in downstream signaling.
Autophosphorylation sites on the epidermal growth factor receptor.
TL;DR: It is reported here that three tyrosine sites near the C-terminus are phosphorylated in vitro in the human EGF receptor, and this site is absent in the v-erb-B protein of avian erythroblastosis virus (AEV), which may influence tyosine kinase activity.
750