Selective Detection of Cu+ Ions in Live Cells via Fluorescence Lifetime Imaging Microscopy.
Martin Priessner,Peter A. Summers,Benjamin W. Lewis,Magdalena Sastre,Liming Ying,Marina K. Kuimova,Ramon Vilar +6 more
TL;DR: In this article, a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions.
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Abstract: Copper is an essential trace element in living organisms with its levels and localisation being carefully managed by the cellular machinery. However, if misregulated, deficiency or excess of copper ions can lead to several diseases. Therefore, it is important to have reliable methods to detect, monitor and visualise this metal in cells. Herein we report a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions. More interestingly, binding to copper(I) leads to significant changes in the fluorescence lifetime of the new probe, which can be used to visualize copper(I) pools in lysosomes of live cells via fluorescence lifetime imaging microscopy (FLIM).
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Recent advances of luminescent sensors for iron and copper: Platforms, mechanisms, and bio-applications
TL;DR: In this article , a mountain of luminescent sensors has been presented for the detection of iron and copper ions, including molecular and nano sensors, including fluorescence, phosphorescence, and bioluminescence.
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Selective Detection of Cu+ Ions in Live Cells via Fluorescence Lifetime Imaging Microscopy.
Martin Priessner,Peter A. Summers,Benjamin W. Lewis,Magdalena Sastre,Liming Ying,Marina K. Kuimova,Ramon Vilar +6 more
TL;DR: In this article, a new optical probe based on BODIPY, which shows a switch-on in its fluorescence intensity upon binding to copper(I), but not in the presence of high concentration of other physiologically relevant metal ions.
51
Oxidation state-specific fluorescent copper sensors reveal oncogene-driven redox changes that regulate labile copper(II) pools
Aidan T. Pezacki,Carson D. Matier,Xingxing Gu,Eric Kummelstedt,Sarah E. Bond,Laura Torrente,Kelly L. Jordan-Sciutto,Gina M. DeNicola,Timothy A Su,Donita C. Brady,Christopher J. Chang +10 more
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Surfactant-Modulated a Highly Sensitive Fluorescent Probe of Fully Conjugated Covalent Organic Nanosheets for Detecting Copper Ions in Aqueous Solution.
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