Rhizobia modulate root-hair-specific expression of extensin genes.
TL;DR: Three cDNAs (ext3, ext127, and ext26), originally isolated by differential screening from a root-hair cDNA library of Vigna unguiculata, were found to encode extensin-like cell wall proteins, and the function of these homologous regions in gene regulation is unknown.
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Abstract: Three cDNAs (ext3, ext127, and ext26), originally isolated by differential screening from a root-hair cDNA library of Vigna unguiculata, were found to encode extensin-like cell wall proteins. Transcripts homologous to these cDNAs were only detected in root hairs where mRNA levels decreased 1 day after inoculation with rhizobia. This coincided with the onset of root-hair deformation, the first morphological step in the Rhizobium-legume interaction. Decreases in transcript levels following inoculation with wild-type Rhizobium sp. NGR234 were more pronounced than with NGRΔnodABC, a mutant deficient in Nod-factor production. Inoculation with a rhizobial strain carrying a mutation in a gene encoding a transcriptional activator for nod genes (NGRΔnodD1) did not repress mRNA levels, indicating that a second nodulation signal may be present that is nodD dependent. Application of purified NodNGR factors only affected transcript levels of ext3. The genomic locus of the gene homologous to ext26 (Ext26G) was cloned. In the 5′ flanking region, several potential TATA boxes and CAP signals were identified. Part of the promoter region shares homology with the Pisum sativum seed lectin promoter and the Nicotiana tabacum nitrate reductase promoter region. Nonetheless, the function of these homologous regions in gene regulation is unknown. Additional keyword: symbiosis.
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Rhizobium sp. strain NGR234 and R. fredii USDA257 share exceptionally broad, nested host ranges.
TL;DR: Both strains nodulated a range of mimosoid legumes, especially the Australian species of Acacia, and the tribe Ingeae, and highest compatibilities were found with the papilionoid tribes Phaseoleae and Desmodieae, suggesting that broad host range originated in Southeast Asia and spread outward.
Plant Cell Wall Remodelling in the Rhizobium–Legume Symbiosis
TL;DR: Root nodule extensins are a legume-specific family of hydroxyproline-rich glycoproteins targeted into the lumen of the infection thread that may serve to regulate fluid-to-solid transitions in the extracellular matrix.
361
Plant gene expression response to Agrobacterium tumefaciens
TL;DR: A rapid plant cell response to Agrobacteria infection is demonstrated, which overlaps a general response to bacteria but also has Agrobacterium-specific features.
206
Expression of AtPRP3, a proline-rich structural cell wall protein from Arabidopsis, is regulated by cell-type-specific developmental pathways involved in root hair formation.
TL;DR: It is demonstrated that AtPRP3, a proline-rich cell wall protein in Arabidopsis, is expressed in root-hair-bearing epidermal cells at the root/shoot junction and within the root differentiation zone of light-grown seedlings, indicating that At PRP3 is regulated by developmental pathways involved in root hair formation.
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Identification of a family of extensin-like glycoproteins in the lumen of rhizobium-induced infection threads in pea root nodules.
TL;DR: Root nodule extensins from pea were of variable length but showed strong sequence conservation of the N- terminus, of the C-terminus, and of a central domain comprising 33 amino acids that were sometimes reiterated.
86
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