Book Chapter10.1016/B978-0-323-91128-3.00020-3
Restriction analysis using NEBcutter
Mohammad Yaseen Sofi,Afshana Shafi,Khalid Z. Masoodi +2 more
- 01 Jan 2022
- pp 159-163
1
About: The article was published on 01 Jan 2022. The article focuses on the topics: Restriction enzyme & Restriction site.
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Citations
Genetic diversity and polymorphism of genes associated with meat quality and growth traits of A Luoi Yellow cattle in Vietnam
Thu Le,Huong Thi Giang Duong,Le Thi Lan Phuong,Thuong Thi Nguyen,T.T. Phan,Le Dinh Phung,Nguyễn Hữu Văn +6 more
TL;DR: This study investigates genetic diversity and polymorphism of genes associated with meat quality and growth traits in A Luoi Yellow cattle, identifying favorable alleles for meat tenderness and body size, and providing insights for future breeding and sustainable development of the breed.
References
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
DNA Restriction Enzyme from E. coli
Matthew Meselson,Robert Yuan +1 more
TL;DR: An endonuclease which degrades foreign DNA has been isolated and requires S-adenosylmethionine, ATP and Mg++.
869
A Restriction enzyme from Hemophilus influenzae: I. Purification and general properties
H O Smith,K W Wilcox +1 more
TL;DR: Extracts of Hemophilus influenzae strain Rd contain an endonuclease activity which produces a rapid decrease in the specific viscosity of a variety of foreign native DNA's; this enzyme was purified approximately 200-fold.
859
NEBcutter: a program to cleave DNA with restriction enzymes
TL;DR: NEBcutter, version 1.0, is a program available via a web server that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence.
REPK: an analytical web server to select restriction endonucleases for terminal restriction fragment length polymorphism analysis.
Roy Eric Collins,Gabrielle Rocap +1 more
TL;DR: REPK (Restriction Endonuclease Picker) assists in the rational choice of restriction endonucleases for T-RFLP by finding sets of four restriction end onucleases that together uniquely differentiate user-designated sequence groups.
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