Reprogramming the genetic code
TL;DR: In this essay, Jason Chin gives an account of his career and of the seminal works on reprogramming translation to generate custom-modified proteins that has gained him the EMBO Gold Medal 2010.
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Abstract: In this essay, Jason Chin gives an account of his career and of the seminal works on reprogramming translation to generate custom-modified proteins that has gained him the EMBO Gold Medal 2010.
read more
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Citations
Expanding the Genetic Code of an Animal
Sebastian Greiss,Jason W. Chin +1 more
TL;DR: The genetic code of a multicellular animal, the nematode Caenorhabditis elegans, is expanded to allow for the site-specific incorporation of unnatural amino acids into proteins.
256
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
Rey W. Martin,Benjamin J. Des Soye,Yong-Chan Kwon,Jennifer E. Kay,Roderick G. Davis,Paul M. Thomas,Natalia I. Majewska,Cindy X. Chen,Ryan D. Marcum,Mary Grace Weiss,Ashleigh E. Stoddart,Miriam Amiram,Arnaz K. Ranji Charna,Jaymin R. Patel,Farren J. Isaacs,Neil L. Kelleher,Seok Hoon Hong,Michael C. Jewett +17 more
TL;DR: A cell-free synthesis method that enables incorporating non-standard amino acids in the product, and the ability to introduce 40 identical p-acetyl-l-phenylalanine residues site specifically into an elastin-like polypeptide, is developed.
202
An Expanded Genetic Code in Mammalian Cells with a Functional Quadruplet Codon
TL;DR: In vitro evolution is utilized to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to a quadruplet codon in both bacterial and mammalian cells, which will allow the biosynthesis of biopolymers that contain multiple unnatural building blocks.
A Facile Strategy for Selective Incorporation of Phosphoserine into Histones
TL;DR: Despite extensive studies, the correlation between phosphorylation and acetylation in histone H3 remains unclear and the development of two opposing models (synergistic and independent) for these modifications is led to.
Site-specific incorporation of photo-cross-linker and bioorthogonal amino acids into enteric bacterial pathogens.
TL;DR: These bioorthogonal functionalities incorporated into enteric pathogens were shown to facilitate the investigation of unique and important proteins involved in the pathogenesis and stress-defense mechanisms of pathogenic bacteria that remain exceedingly difficult to study using conventional methodologies.
93
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