Reprogramming microbial populations using a programmed lysis system to improve chemical production.
Wenwen Diao,Liang Guo,Qiang Ding,Cong Gao,Guipeng Hu,Xiulai Chen,Yang Li,Linpei Zhang,Wei Chen,Jian Chen,Liming Liu +10 more
23
TL;DR: In this article, a programmed lysis system (PLS) was developed to reprogram microbial cooperation to enhance chemical production, where a colicin M -based lysis unit was constructed to lyse Escherichia coli.
read more
Abstract: Microbial populations are a promising model for achieving microbial cooperation to produce valuable chemicals. However, regulating the phenotypic structure of microbial populations remains challenging. In this study, a programmed lysis system (PLS) is developed to reprogram microbial cooperation to enhance chemical production. First, a colicin M -based lysis unit is constructed to lyse Escherichia coli. Then, a programmed switch, based on proteases, is designed to regulate the effective lysis unit time. Next, a PLS is constructed for chemical production by combining the lysis unit with a programmed switch. As a result, poly (lactate-co-3-hydroxybutyrate) production is switched from PLH synthesis to PLH release, and the content of free PLH is increased by 283%. Furthermore, butyrate production with E. coli consortia is switched from E. coli BUT003 to E. coli BUT004, thereby increasing butyrate production to 41.61 g/L. These results indicate the applicability of engineered microbial populations for improving the metabolic division of labor to increase the efficiency of microbial cell factories. Microbial ecosystem-based bioproduction requires the regulation of phenotypic structure of microbial populations. Here, the authors report the construction of a programmed lysis system and its ability for reprograming microbial cooperation in poly(lactate-co-3-hydroxybutyrate) and butyrate production by E. coli strains.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Genetically encoded biosensors for microbial synthetic biology: From conceptual frameworks to practical applications.
TL;DR: In this paper , the authors provided theoretical guidance for constructing genetically encoded biosensors to create desirable microbial cell factories for sustainable bioproduction, and discussed the challenges and prospects for constructing robust and sophisticated biometric sensors.
40
Guided by the principles of microbiome engineering: Accomplishments and perspectives for environmental use
Haiyang Hu,Miaoxiao Wang,Yiqun Huang,Zhaoyong Xu,Ping Xu,Yong Nie,Hongzhi Tang +6 more
TL;DR: This review highlights recent microbiome engineering strategies for bioremediation, combining top-down and bottom-up approaches to address environmental challenges, such as refractory pollutant degradation and sustaining performance under fluctuating conditions.
30
Improvement in bacterial cellulose production by co-culturing Bacillus cereus and Komagataeibacter xylinus.
Wenchao Li,Xinxin Huang,Huan-xin Liu,Haojun Lian,Xue Sun,Wei Wang,Shiru Jia,Cheng Zhong +7 more
TL;DR: In this paper , a co-cultured Bacillus cereus and Komagataeibacter xylinus was found to increase the BC yield in corn stover enzymatic hydrolysate from 1.2 to 4.4 g/L.
17
Relieving metabolic burden to improve robustness and bioproduction by industrial microorganisms
Jiwei Mao,H Zhang,Chen Yu,Wenju Liang,Jun Liu,Jens Nielsen,Yun Chen,Nianjun Xu +7 more
Abstract: Metabolic burden is defined by the influence of genetic manipulation and environmental perturbations on the distribution of cellular resources. The rewiring of microbial metabolism for bio-based chemical production often leads to a metabolic burden, followed by adverse physiological effects, such as impaired cell growth and low product yields. Alleviating the burden imposed by undesirable metabolic changes has become an increasingly attractive approach for constructing robust microbial cell factories. In this review, we provide a brief overview of metabolic burden engineering, focusing specifically on recent developments and strategies for diminishing the burden while improving robustness and yield. A variety of examples are presented to showcase the promise of metabolic burden engineering in facilitating the design and construction of robust microbial cell factories. Finally, challenges and limitations encountered in metabolic burden engineering are discussed.
13
Enhancing tumor-specific recognition of programmable synthetic bacterial consortium for precision therapy of colorectal cancer
Tuoyu Zhou,Jingyuan Wu,Haibo Tang,Dali Liu,Byong-Hun Jeon,Weilin Jin,Yiqing Wang,Yuanzhang Zheng,Aman Khan,Huawen Han,Xiangkai Li +10 more
TL;DR: Genetic circuits based on the E. coli Nissle 1917 chassis were developed to sense indicators of tumor microenvironment and control the expression of therapeutic payloads, and synthetic bacterial consortium (SynCon) served to augment the synergistic effect of this approach.
11
References
Pyrosequencing enumerates and contrasts soil microbial diversity
Luiz Fernando Wurdig Roesch,Roberta R. Fulthorpe,Alberto Riva,George Casella,Alison K M Hadwin,Angela D. Kent,Samira H. Daroub,Flávio Anastácio de Oliveira Camargo,William G. Farmerie,Eric W. Triplett +9 more
TL;DR: This work is the most comprehensive examination to date of bacterial diversity in soil and suggests that agricultural management of soil may significantly influence the diversity of bacteria and archaea.
Identification and characterization of essential genes in the human genome
Timothy C. Wang,Kıvanç Birsoy,Nicholas W. Hughes,Kevin M. Krupczak,Yorick Post,Yorick Post,Jenny J. Wei,Eric S. Lander,Eric S. Lander,Eric S. Lander,David M. Sabatini +10 more
TL;DR: Using the bacterial clustered regularly interspaced short palindromic repeats (CRISPR) system, this article constructed a genome-wide single-guide RNA library to screen for genes required for proliferation and survival in a human cancer cell line.
1.6K
Engineering microbial consortia: a new frontier in synthetic biology.
TL;DR: Recent efforts to engineer synthetic microbial consortia are reviewed, and future applications are suggested to suggest future applications.
1K
Microbial population structures in the deep marine biosphere.
Julie A. Huber,David B. Mark Welch,Hilary G. Morrison,Susan M. Huse,Phillip R. Neal,David A. Butterfield,Mitchell L. Sogin +6 more
TL;DR: It is predicted that hundreds of thousands of sequences will be necessary to capture the vast diversity of microbial communities, and that different patterns of evenness for both high- and low-abundance taxa may be important in defining microbial ecosystem dynamics.
Type VI secretion system effectors: poisons with a purpose
TL;DR: The type VI secretion system (T6SS) mediates interactions between a broad range of Gram-negative bacterial species and has been shown to be involved in both antagonistic and non-antagonistic behaviors as mentioned in this paper.