Journal Article10.1016/J.MBS.2013.05.007
Regulatory volume decrease of rat kidney principal cells after successive hypo-osmotic shocks
Sotirios G. Zarogiannis,A. V. Ilyaskin,G. S. Baturina,Liubov E. Katkova,Dmitriy A. Medvedev,Dmitriy A. Medvedev,Denis I. Karpov,Denis I. Karpov,A. P. Ershov,A. P. Ershov,Evgeniy I. Solenov,Evgeniy I. Solenov +11 more
TL;DR: A sub-second temporal analysis of RVD events occurring after two successive hypo-osmotic challenges in rat kidney OMCD principal cells is provided and a mathematical model is created based on experimental results to provide the basis for further detailed studies on RVD dynamical modeling.
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Abstract: Outer Medullary Collecting Duct (OMCD) principal cells are exposed to significant changes of the extracellular osmolarity and thus the analysis of their regulatory volume decrease (RVD) function is of great importance in order to avoid cell membrane rupture and subsequent death. In this paper we provide a sub-second temporal analysis of RVD events occurring after two successive hypo-osmotic challenges in rat kidney OMCD principal cells. We performed experimental cell volume measurements and created a mathematical model based on our experimental results. As a consequence of RVD the cell expels part of intracellular osmolytes and reduces the permeability of the plasma membrane to water. The next osmotic challenge does not cause significant RVD if it occurs within a minute after the primary shock. In such a case the cell reacts as an ideal osmometer. Through our model we provide the basis for further detailed studies on RVD dynamical modeling.
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Citations
Quantitative estimation of transmembrane ion transport in rat renal collecting duct principal cells.
A. V. Ilyaskin,Denis I. Karpov,Dmitriy A. Medvedev,A. P. Ershov,G. S. Baturina,Liubov E. Katkova,Evgeniy I. Solenov +6 more
TL;DR: This approach sets grounds for utilization of experimental measurements of intracellular sodium concentration and cell volume to quantify the ion permeabilities of OMCD principal cells and aids us in understanding the physiology of the adjustment of renal sodium and potassium excretion.
The water permeability reduction after successive hypo-osmotic shocks in kidney principal cells is apically regulated.
TL;DR: The results indicate that in principal cells the effective mechanism of RVD is mainly regulated by the apical cell plasma membrane.
Methods to Measure Water Permeability
TL;DR: This chapter describes and critically assess several methods that have been developed for the measurement of water permeability both in living cells as well as in tissues with a focus in the first category.
7
Benign Pleural Mesothelial Cells Have Higher Osmotic Water Permeability than Malignant Pleural Mesothelioma Cells and Differentially Respond to Hyperosmolality.
Liubov E. Katkova,G. S. Baturina,Alexander A. Bondar,Rajesh Jagirdar,Chrissi Hatzoglou,Konstantinos I. Gourgoulianis,Evgeniy I. Solenov,Sotirios G. Zarogiannis +7 more
TL;DR: Comparing cell lines after high glucose preconditioning, MeT-5A Pf was significantly higher than that of M14K and ZL34 MPM cells and the AQP1 inhibition effect was significant in MeT/M14K cells.
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