Journal Article10.1007/S10681-007-9482-7
RAPD markers linked to a rust resistance gene in cultivated groundnut (Arachis hypogaea L.)
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TL;DR: Marker J71300 identified all homozygous rust resistant genotypes in the F2 population and was present in all the eight susceptible genotypes tested for validation, which should be applicable for marker-assisted selection in the groundnut rust resistance breeding programme in India.
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Abstract: Groundnut rust (Puccinia arachidis Speg.) is an important air borne pathogen, which causes substantial losses in groundnut yield and quality. Although large numbers of accessions were identified as rust resistant in wild, interspecific derivative and cultivated groundnut species, transfer of resistance to well-adapted cultivars is limited due to linkage drag, which worsens yield potential and market acceptance. A F2 mapping population comprising 117 individuals was developed from a cross between the rust resistant parent VG 9514 and rust susceptible parent TAG 24. Rust resistance was governed by single dominant gene in this cross. We identified 11 (out of 160) RAPD primers that exhibited polymorphism between these two parents. Using a modified bulk segregant analysis, primer J7 (5′CCTCTCGACA3′) produced a single coupling phase marker (J71350) and a repulsion phase marker (J71300) linked to rust resistance. Screening of the entire F2 population using primer J7 revealed that the coupling phase marker J71350 was linked with the rust resistance gene at a distance of 18.5 cM. On the other hand, the repulsion phase marker J71300 was completely linked with rust resistance. Additionally, both J71300 (P = 0.00075) and J71350 (P < 0.00001) were significantly associated with the rust resistance. Marker J71300 identified all homozygous rust resistant genotypes in the F2 population and was present in all the eight susceptible genotypes tested for validation. Thus, J71300 should be applicable for marker-assisted selection (MAS) in the groundnut rust resistance breeding programme in India. To the best of our knowledge this is the first report on the identification of RAPD markers linked to rust resistance in groundnut.
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Citations
Identification of quantitative trait loci for protein content, oil content and oil quality for groundnut (Arachis hypogaea L.)
Cholin Sarvamangala,Cholin Sarvamangala,Cholin Sarvamangala,M. V. C. Gowda,Rajeev K. Varshney,Rajeev K. Varshney +5 more
TL;DR: An attempt was made to identify microsatellite or simple sequence repeat (SSR) markers for important nutritional traits in a mapping population consisting of 146 recombinant inbreed lines (RILs) of a cross TG26 × GPBD4, and negative correlation between protein content and oil content, oleic acid and linoleic acid indicated their antagonistic nature.
180
Breeding Major Oil Crops: Present Status and Future Research Needs
Devendra K. Yadava,Sujata Vasudev,Naveen Singh,T. Mohapatra,Kumble Vinod Prabhu +4 more
- 01 Jan 2012
TL;DR: In this paper, a review describes developments in use of biotechnological tools in seven edible oil crops, namely, Brassica, soybean, sunflower, groundnut, Sesamum, linseed and safflower and also highlights the prospects of using markers in genetic improvement of these crops.
71
Molecular diversity and association of SSR markers to rust and late leaf spot resistance in cultivated groundnut (Arachis hypogaea L.)
TL;DR: Molecular diversity and association of simple sequence repeat (SSR) markers with rust and late leaf spot (LLS) resistance were detected in a set of 20 cultivated groundnut genotypes differing in resistance against both diseases.
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SSR markers associated for late leaf spot disease resistance by bulked segregant analysis in groundnut ( Arachis hypogaea L.)
TL;DR: PM 384 could be utilized in the marker assisted breeding programme over a wide range of genetic background as it is able to distinguish the resistant and susceptible bulks and individuals for LLS.
Development of genic molecular markers linked to a rust resistance gene in cultivated groundnut (Arachis hypogaea L.)
TL;DR: Through genetic mapping, EST-SSR markers SSR_GO340445 and SSR-HO115759 were found closely linked to a rust resistance gene at 1.9 and 3.8 cM distances, respectively, and are suitable candidates for marker assisted selection in groundnut.
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