Journal Article10.1002/9780470151808.SC01E07S35
Quantifying Epithelial Early Common Progenitors from Long-Term Primary or Cell Line Sphere Culture.
Flora Clément,Flora Clément,Flora Clément,Helen He Zhu,Wei-Qiang Gao,Emmanuel Delay,Véronique Maguer-Satta +6 more
1
TL;DR: This protocol is based on the combination of two functional tests: the sphere assay to maintain and enrich early progenitor/stem cells, and the epithelial colony-forming cells (E-CFC) assay to identify and quantify further differentiated epithelial progenitors.
read more
Abstract: Here, a protocol to quantify epithelial early common progenitor/stem cells grown as spheres in non-adherent culture conditions is described. This protocol is based on the combination of two functional tests: the sphere assay to maintain and enrich early progenitor/stem cells, and the epithelial colony-forming cells (E-CFC) assay to identify and quantify further differentiated epithelial progenitors. Primary spheres mainly contain progenitors and rare stem/early common progenitor cells while secondary and tertiary spheres contain progenitor cells derived from the early common progenitor/stem cell population maintained through passages and partially differentiated. Spheres are enzymatically and mechanically dissociated; the derived cells are subsequently plated on irradiated NIH-3T3 fibroblasts for further processing, as in the E-CFC assay. The principle of this assay is to quantify the number of epithelial colonies generated by cells present in the different sequential spheres. This assay has therefore been named the early common progenitor-derived colonies assay (ECP-DC).
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Long-term exposure to bisphenol A or benzo(a)pyrene alters the fate of human mammary epithelial stem cells in response to BMP2 and BMP4, by pre-activating BMP signaling.
Flora Clément,Xinyi Xu,Caterina F Donini,Alice Clement,Soleilmane Omarjee,Emmanuel Delay,Isabelle Treilleux,Béatrice Fervers,Muriel Le Romancer,Pascale A. Cohen,Véronique Maguer-Satta +10 more
TL;DR: It is demonstrated that chronic exposure to low doses of bisphenol A or benzo( a)pyrene (B(a)P) alone has little effect on SCs properties of MCF10A cells, and that BPA prevents the maintenance of SC features prompted by BMP4, whereas promoting cell differentiation towards a myoepithelial phenotype is prevented.
References
In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells
Gabriela Dontu,Wissam M. Abdallah,Jessica M. Foley,Kyle W. Jackson,Michael F. Clarke,Mari J. Kawamura,Max S. Wicha +6 more
TL;DR: It is demonstrated that nonadherent mammospheres are enriched in early progenitor/stem cells and able to differentiate along all three mammary epithelial lineages and to clonally generate complex functional structures in reconstituted 3D culture systems.
Phenotypic and functional characterization in vitro of a multipotent epithelial cell present in the normal adult human breast
TL;DR: The phenotypic profile of these cells suggest that these multilineage HBEC progenitors are a relatively undifferentiated cell since they express low levels of MUC-1 and that they have a luminal location within the mammary epithelium since they are ESA+.
264
Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo.
Isla P. Garraway,Wenyi Sun,Chau P. Tran,Sven Perner,Bao Zhang,Andrew S. Goldstein,Scott Hahm,Maahum Haider,Christian Head,Robert E. Reiter,Mark A. Rubin,Owen N. Witte +11 more
TL;DR: A subset of human prostate cells that form prostaspheres were evaluated for self‐renewal and tissue regeneration capability in the present study.
157
Stem Cell Characteristics in Prostate Cancer Cell Lines
TL;DR: In this paper, the authors examined six established prostate cancer (PCa) cell lines (DU145, DuCaP, LAPC-4, 22Rv1, LNCaP, and PC-3) for their stem cell properties in vitro.
124
Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response.
Marion Chapellier,Elodie Bachelard-Cascales,Xenia Schmidt,Flora Clément,Isabelle Treilleux,Emmanuel Delay,Alexandre Jammot,Christine Ménétrier-Caux,Gaëtan Pochon,Roger Besançon,Thibault Voeltzel,Claude Caron de Fromentel,Christophe Caux,Jean-Yves Blay,Richard Iggo,Véronique Maguer-Satta +15 more
TL;DR: A role for BMP2 and the breast microenvironment in the initiation of stem cell transformation is revealed, thus providing insight into the etiology of luminal breast cancer.
66