Pycro-Manager: open-source software for customized and reproducible microscope control.
Henry Pinkard,Nico Stuurman,Ivan Ivanov,Nicholas M. Anthony,Wei Ouyang,Bin Li,Bin Yang,Mark A. Tsuchida,Bryant B. Chhun,Grace Zhang,Ryan Mei,Michael W. Anderson,Michael W. Anderson,Douglas P. Shepherd,Ian Hunt-Isaak,Raymond L. Dunn,Wiebke Jahr,Saul Kato,Loic Royer,Jay R. Thiagarajah,Jay R. Thiagarajah,Kevin W. Eliceiri,Emma Lundberg,Shalin B. Mehta,Laura Waller +24 more
About: This article is published in Nature Methods. The article was published on 05 Mar 2021. and is currently open access.
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Citations
Light sheet fluorescence microscopy
Ernst H. K. Stelzer,Frederic Strobl,Bo-Jui Chang,Friedrich Preusser,Stephan Preibisch,Katie McDole,Reto Fiolka +6 more
- 03 Nov 2021
TL;DR: Light sheet fluorescence microscopy (LSFM) is a technique that uses a thin sheet of light for illumination, allowing optical sectioning of the sample as discussed by the authors, and is used for in-depth analyses of large, optically cleared samples and long-term three-dimensional (3D) observations of live biological specimens at high spatio-temporal resolution.
173
DaXi—high-resolution, large imaging volume and multi-view single-objective light-sheet microscopy
Bin Yang,Merlin Lange,Alfred Millett-Sikking,Xiangye Zhao,Jordão Bragantini,Shruthi VijayKumar,Mason Kamb,R. Gómez-Sjöberg,Ahmet Can Solak,Wanpeng Wang,Hirofumi Kobayashi,Matthew N. McCarroll,Lachlan Whitehead,Reto Fiolka,Thomas B. Kornberg,Andrew York,Loic Royer +16 more
TL;DR: DaXi as discussed by the authors is a single-objective light-sheet microscopy design based on oblique plane illumination that achieves a wider field of view and high-resolution imaging via a custom remote focusing objective.
Light-sheets and smart microscopy, an exciting future is dawning
Stephan Daetwyler,Reto Fiolka +1 more
TL;DR: In this article , the authors discuss current and future developments in light-sheet fluorescence microscopy that they expect to further expand its capabilities, including smart and adaptive imaging schemes to overcome traditional imaging trade-offs, i.e., spatiotemporal resolution, field of view and sample health.
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Open microscopy in the life sciences: Quo Vadis?.
Johannes Hohlbein,Madeline Yung,Benedict Diederich,Barbora Marsikova,Emmanuel G. Reynaud,Seamus Holden,Wiebke Jahr,Robert Haase,Kirti Prakash +8 more
TL;DR: In this article, the authors argue that the availability of well-designed open hardware and software solutions targeting broad user groups or even non-experts will increasingly be relevant to cope with the increasing complexity of cutting-edge imaging technologies.
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Isotropic imaging across spatial scales with axially swept light-sheet microscopy
Tao Chen,Mohamed Benabid +1 more
TL;DR: In this paper , the axially swept light-sheet microscopy (ASLM) is described and a detailed part list and schematics for several variants of ASLM are provided.
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Advanced methods of microscope control using μManager software.
Arthur D. Edelstein,Mark A. Tsuchida,Nenad Amodaj,Henry Pinkard,Ronald D. Vale,Nico Stuurman +5 more
- 07 Nov 2014
TL;DR: A guide to using some of the recently added advanced μManager features, including hardware synchronization, simultaneous use of multiple cameras, projection of patterned light onto a specimen, live slide mapping, imaging with multi-well plates, particle localization and tracking, and high-speed imaging.