Purification and characterization of a plant antimicrobial peptide expressed in Escherichia coli.

TL;DR: A review of commonly used fusion partners (e.g., solubility-enhancing, aggregation-promoting and self-cleavable carriers, etc.), cleavage methods and optimization options for antimicrobial peptides production in E. coli is presented in this paper.

TL;DR: Data revised here indicate that large-scale production of AMPs can be obtained using biotechnological tools, and the products may be applied in the pharmaceutical industry as well as in agribusiness.

TL;DR: This work has identified peptides that play crucial roles in plant growth and development, including defence mechanisms in response to wounding by pests, the control of cell division and expansion, and pollen self-incompatibility.

TL;DR: In this paper, the most commonly used carrier proteins for fusion expression of antimicrobial peptides in E. coli were considered and SUMO (small ubiquitin-related modifier) was proposed as a novel fusion partner.

TL;DR: A gene expression system based on bacteriophage T7 RNA polymerase has been developed and high levels of accumulation suggest that the RNAs are relatively stable, perhaps in part because their great length and/or stem-and-loop structures at their 3' ends help to protect them against exonucleolytic degradation.

TL;DR: A novel approach to tailored selective excitation for the measurement of NMR spectra in non-deuterated aqueous solutions, which effectively combines one selective 180° radiofrequency pulse and two field gradient pulses, achieves highly selective and effective water suppression.

TL;DR: The results indicate that systemic pathogen-induced expression of the plant defensin gene in Arabidopsis is independent of salicylic acid but requires components of the ethylene and jasmonic acid response.