Journal Article10.1016/S0956-5663(02)00147-1
Protein patterning on silicon-based surface using background hydrophobic thin film
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TL;DR: A new and convenient protein patterning method on silicon-based surface was developed for protein array by spin coating of hydrophobic thin film (CYTOP), and quantitative analysis of the protein binding was demonstrated by streptavidin-biotin system.
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About: This article is published in Biosensors and Bioelectronics. The article was published on 01 Apr 2003. The article focuses on the topics: Contact angle.
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Citations
Development and application of diazirines in biological and synthetic macromolecular systems
Anton Blencowe,Wayne Hayes +1 more
TL;DR: The use of diazirines as stable carbene precursors has increased dramatically over the past twenty years and these reagents are fast becoming the most popular photophors for photoaffinity labeling and biological applications in which covalent modification of macromolecular structures is the basis to understand structure-activity relationships as mentioned in this paper.
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•Journal Article
Development and application of diazirines in biological and synthetic macromolecular systems
Anton Blencowe,Wayne Hayes +1 more
TL;DR: The use of diazirines as stable carbene precursors has increased dramatically over the past twenty years and these reagents are fast becoming the most popular photophors for photoaffinity labelling and biological applications in which covalent modification of macromolecular structures is the basis to understanding structure-activity relationships.
114
Polymeric Brushes as Functional Templates for Immobilizing Ribonuclease A: Study of Binding Kinetics and Activity
TL;DR: Poly(acrylic acid) brushes are employed as templates to immobilize ribonuclease A (RNase A), which is commonly used to remove RNA from plasmid DNA preparations, and covalent esterification resulted in similar temperature dependence as free enzyme, whereas metal-ion complexation showed no temperature dependence indicating a significant change in conformation.
107
A surface modification strategy on silicon nitride for developing biosensors
TL;DR: HrpW in these protein mixtures was successfully recognized by anti-HrPW antibodies when mixed proteins were immobilized onto activated surfaces, providing a platform onto which proteins can be directly immobilized for biosensor and protein array applications.
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Surface-anchored poly(2-vinyl-4,4-dimethyl azlactone) brushes as templates for enzyme immobilization.
TL;DR: The PVDMA template was extended to other biotechnologically relevant enzymes, such as deoxyribonuclease I, glucose oxidase, glucoamylase, and trypsin, with relative activities higher than or comparable to those of enzymes immobilized by other means.
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Patterning proteins and cells using soft lithography
TL;DR: This review describes the pattering of proteins and cells using a non-photolithographic microfabrication technology, which consists of a set of related techniques, each of which uses stamps or channels fabricated in an elastomeric ('soft') material for pattern transfer.
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Fabrication of submicrometer features on curved substrates by microcontact printing
TL;DR: Wet-chemical etching that uses the patterned SAMs as resists transfers the patterns formed by mu CP into gold, and there is no comparable method for microfabrication on curved surfaces.
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Covalent immobilization of protein monolayers for biosensor applications
R.A. Williams,Harvey W. Blanch +1 more
TL;DR: It was determined that approximately 75% of the protein on the "covalently" immobilized samples was simply adsorbed, and this surface density is consistent with a monolayer of immobilized protein.
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Aggregation of HSA, IgG, and Fibrinogen on Methylated Silicon Surfaces
TL;DR: The main conclusion that can be extracted from this work is that fibrinogen interacts more irreversibly with the silicon surfaces than IgG, and much more so than HSA, which is less hydrophobic and smaller than fibr inogen.
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