Production of transgenic broilers by non-viral vectors via optimizing egg windowing and screening transgenic roosters.
Zhong-Bin Wang,Zhi-Qiang Du,Wei Na,Jun-Hong Jing,Yumao Li,Li Leng,Peng Luan,Chun-Yan Wu,Ke Zhang,Yuxiang Wang,Wen-Li Liu,Hui Yuan,Zhonghua Liu,Yan-Shuang Mu,Qing-Wen Meng,Ning Wang,Cai-Xia Yang,Hui Li +17 more
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TL;DR: It is discovered that proper manipulation of the inner shell membrane at the blunt end could improve the rate of producing G0 transgenic roosters and appropriate egg windowing and screening of potentially transgene‐positive rooster can improve the production of germline‐transmitted transgenic birds.
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About: This article is published in Poultry Science. The article was published on 01 Jan 2019. and is currently open access.
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Common Gene Modules Identified for Chicken Adiposity by Network Construction and Comparison.
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TL;DR: The lysosome pathway was validated, and found TFEB and its downstream target genes showed similar expression patterns along with chicken preadipocyte differentiation, which could provide novel insights into the genetic basis of complex adiposity traits, as well as human obesity and related metabolic diseases.
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Transgenesis and genome editing in chickens
Xiaofei Wang,Laruen E. Shields,Rebecca L. Welch,Alexis Pigg,Karim Kaleh +4 more
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TL;DR: This chapter reviews the history and current state of chicken genome manipulation, with specific focus on recent developments in the field, including review of currently available tools and resources for genome manipulation based on discoveries in recombinant DNA technology and embryonic engineering.
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The science of genetically modified poultry
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Study on the Efficient Methods for Chicken Eggshell windowing and the Factors Affecting the Hatchability
TL;DR: In order to find out the main factors influenced the hatchability and improve the hatch ability of the windowed chicken eggs at stage X, several experiments were made on the basis of the former patent of eggshell windowing methods on equatorial plane, such as cutting and sealing techniques, air cell recovering, laying position immediately after sealing, as well as the injection volumes into the subgerminal cavity of the blastoderm as mentioned in this paper.
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