Open AccessJournal Article
Production and characterization of phytase from Bacillus spp. as feed additive in aquaculture
TL;DR: In this article, four species of Bacillus, namely, B. pumilus, b. megaterium, coagulans, and B. licheniformis were used to study the biochemical characteristics of their phytases.
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Abstract: Phytases are phosphohydrolases that catalyze the release of phosphate from phytate (myo- inositol hexakisphosphate), the major phosphorus (P) form mostly occurring in animal feeds of plant origin. These enzymes can be supplemented in animal diets to reduce inorganic phosphorus supplementation and fecal phosphorus excretion. Four species of Bacillus namely, B. pumilus, B. megaterium, B. coagulans, and B. licheniformis were used to study the biochemical characteristics of their phytases. All the strains investigated were able to hydrolyze extracellular phytate. The activity of phytase increased markedly at the late stationary phase in all the species tested. Highest enzyme activity was found in phytase from B. megaterium after the 4 th day of culture. The crude phytases from the different Bacillus strains were optimally active at pH values ranging 5.5 to 7.0 at 37 0 C and retained their activity at temperatures up to 80 0 C. The enzymes exhibited thermostability, retaining ~50 % activity at 70 0 C and were fairly stable up to pH 10. These properties indicate that the Bacillus phytases
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Citations
Microbial production of phytases for combating environmental phosphate pollution and other diverse applications
TL;DR: Recent advances in microbial phytase production, application of tools to optimize higher enzyme production, and characterization of phytases along with potential biotechnological applications are reviewed.
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Phytase: The Feed Enzyme, an Overview
Namita Singh,Sonia Kuhar,Kanu Priya,Rajneesh Jaryal,Rakesh Yadav +4 more
- 01 Jan 2018
TL;DR: A wide and comprehensive discussion of the phytase, which performs a pivotal function in biochemistry of inositol phosphates, is covered herein along with cataloguing a number of sources that produce the enzyme.
15
Purification and Characterization of Extracellular Phytase from Bacillus licheniformis Isolated from Fish Gut
Suhas Kumar Dan,Ankita Nandi,Goutam Banerjee,Pinki Ghosh,Arun Kumar Ray +4 more
- 27 Jun 2015
TL;DR: The properties of the presently purified phytase to hydrolyze plant phytate and maintaining stability at high temperature make it suitable for applications in animal feed industry.
10
•Journal Article
The potential use of legume-based diets supplemented with microbial phytase on the growth performance and feed efficiency of sea bass, Lates calcarifer.
TL;DR: Results from the present study showed that incorporation of dietary microbial phytase in legume based diets slightly improve the growth performance and P availability in sea bass juveniles.
6
References
•Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Phytates in Legumes and Cereals
TL;DR: This chapter discusses that the knowledge of phytic acid had its beginning in the discovery by Hartig, who isolated small particles or grains from the seeds of various plants, and the correlation of phytate with the cooking quality of peas was first suggested by Mattson.
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Simple and rapid determination of phytase activity.
TL;DR: A simple and rapid method is described for determining the enzymatic activity of microbial phytase based on the determination of inorganic orthophosphate released on hydrolysis of sodium phytate at pH 5.5.
567
Purification and Characterization of Two Phytases from Escherichia coli
TL;DR: The chemical and kinetic properties of the purified phytase P2 points to an identity with an enzyme described by Dassa et al. (1982) as a pH 2.5 acid phosphatase; consequently, this enzyme is a 6-phytase.
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Influence of Dietary Calcium, Phosphorus, Zinc and Sodium Phytate Level on Cataract Incidence, Growth and Histopathology in Juvenile Chinook Salmon (Oncorhynchus tshawytscha)
TL;DR: It is concluded that zinc is essential for normal eye development in juvenile chinook Salmon and zinc deficiency could not be induced in chinook salmon fed diets with high ratios of calcium (or phosphorus) to zinc alone.
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